| 解淀粉芽孢杆菌B9601-Y2对玉米的防病促生长效果研究 |
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| 引用本文： | 崔文艳,何朋杰,尚娟,李艳云,吴毅歆,何月秋.解淀粉芽孢杆菌B9601-Y2对玉米的防病促生长效果研究[J].玉米科学,2015,23(5):153-158. |
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| 作者姓名： | 崔文艳 何朋杰 尚娟 李艳云 吴毅歆 何月秋 |
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| 作者单位： | 1. 云南农业大学植物保护学院, 昆明 650201;2. 微生物菌种筛选与应用国家地方联合工程研究中心, 昆明 650207,1. 云南农业大学植物保护学院, 昆明 650201;2. 微生物菌种筛选与应用国家地方联合工程研究中心, 昆明 650207,微生物菌种筛选与应用国家地方联合工程研究中心, 昆明 650207,微生物菌种筛选与应用国家地方联合工程研究中心, 昆明 650207,1. 微生物菌种筛选与应用国家地方联合工程研究中心, 昆明 650207;2. 云南农业大学农学与生物技术学院, 昆明 650201,1. 微生物菌种筛选与应用国家地方联合工程研究中心, 昆明 650207;2. 云南农业大学农学与生物技术学院, 昆明 650201 |
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| 基金项目： | 科技部国际合作项目(2009DFA32360)、云南省玉米产业体系项目 |
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| 摘 要： | 通过平板对峙培养及盆栽试验,研究解淀粉芽孢杆菌B9601-Y2(Y2)防治玉米小斑病和茎基腐病以及提高玉米生物量的效果。平板对峙培养结果表明,Y2发酵液、无菌滤液及菌体悬液对玉米小斑病菌和茎基腐病原菌均有拮抗作用。盆栽实验结果显示,3种处理对玉米小斑病防效60%左右,对茎腐病防效50%左右,防效依次为Y2发酵液菌体悬液无菌滤液。单独使用Y2发酵液、无菌滤液及菌体悬液处理的苗根长、株高、鲜重和干重均显著高于清水和病原菌对照20%以上,喷施或灌溉接种病原菌的玉米苗生长量显著高于单独接种病原菌处理,有效消除了病原菌对植株生长的影响。因此,生防菌Y2具有用于防治玉米病害和具有根肥及叶面肥的潜力。
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| 关 键 词： | 玉米 生防菌 拮抗 小斑病 茎基腐病 |
| 收稿时间： | 3/6/2015 12:00:00 AM |
Effects of Bacillus amyloliquefaciens B9601-Y2 on Diseases Control and Growth-promotion of Maize |
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| CUI Wen-yan,HE Peng-jie,SHANG Juan,LI Yan-yun,WU Yi-xin and HE Yue-qiu.Effects of Bacillus amyloliquefaciens B9601-Y2 on Diseases Control and Growth-promotion of Maize[J].Journal of Maize Sciences,2015,23(5):153-158. |
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| Authors: | CUI Wen-yan HE Peng-jie SHANG Juan LI Yan-yun WU Yi-xin and HE Yue-qiu |
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| Institution: | 1. Faculty of Plant Protection, Yunnan Agricultural University, Kunming 650201;2. National and Local Joint Engineering Research Center for Screening and Application of Microbial Strains, Kunming 650207,1. Faculty of Plant Protection, Yunnan Agricultural University, Kunming 650201;2. National and Local Joint Engineering Research Center for Screening and Application of Microbial Strains, Kunming 650207,National and Local Joint Engineering Research Center for Screening and Application of Microbial Strains, Kunming 650207,National and Local Joint Engineering Research Center for Screening and Application of Microbial Strains, Kunming 650207,1. National and Local Joint Engineering Research Center for Screening and Application of Microbial Strains, Kunming 650207;2. Faculty of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China and 1. National and Local Joint Engineering Research Center for Screening and Application of Microbial Strains, Kunming 650207;2. Faculty of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China |
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| Abstract: | By dual-culture confrontation assay(DCA) and pot culture test, to study the effect of Bacillus amyloliquefaciens B9601-Y2(Y2) to control southern leaf blight(SLB) and stalk rot and increase biomass of maize. DCA showed that formulate suspension(FS), cell-free filtrate(CF) and cell suspension(CS) of Y2 could inhibit mycelial growth of Bipolaris maydis and Fusarium verticillioides. Pot culture test indicated that those suspensions could control over 60% of SLB and over 50% of stalk rot with the tendency FS > CS > CF. Those suspensions promoted over 20% of root length, plant type height, dry weight of above-ground and dry weight of under-ground for healthy maize and for the disease seedlings inoculated with B. maydis and F. verticillioides by spray or drenching. Therefore, Y2 has the potential serving as a root and leaf biofertilizer with disease control effect for maize. |
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| Keywords: | Maize Biocontrol agent Antagonism Southern leaf blight Stalk rot |
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