非一般培养亲件下瘤胃液质量培养基质对培养液pH值的影响

非一般体外培养条件下，用Ｌ４２（３）正交设计研究培养瓶中瘤胃液质量，培养基质中能量和蛋白质对培养液ｐＨ值的影响。结果表明：１．影响培养液ｐＨ值的主要因素是基质。量大时ｐＨ下降得快。其中的蛋白质和能量在不同时间发挥作用的地位不同。２．培养液中微生物首先利用基质中能量物质生长，同时分解蛋白质；然后利用基质中能量和蛋白质生长繁殖，在合成微生物蛋白的同时导致ｐＨ值下降；最后利用能量维持自身。３．瘤胃液质量也即瘤胃液微生物区系和其活性也影响培养液ｐＨ值，并在不同的时间发挥不同性质的作用。说明生产上饲喂动物应有合理的饲喂方法。

The Influence of Rumenfluid quality and Cultural Substance to pH Value of Cultivated Content in Vitro Under an Unordinary Condition

The influence of rumenfluid quality and cultural substance to pH value of cultivated content in vitro under an unordinary condition was investigated in a L42(3) square experiment.The results showed that:1.Main factor to pH value of cultivated content was cultural substance, as its quantity increased PH value decreased fast and protein and energy of it had different effect on pH value in different time.2. At first, microbes grew with energy and cultural protein decomposed,then microbes grew and bred with protein and erengy, resulted decrease of PH value as microbial protein synthesized, at last microbes maintained themselves with energy.3.The quality of rumenfluid -rumenfluid microflue and its activity-also had an effect on cultivated content PH value and had an opposite characteristic in different cultivated time.The conclusion is that the reasonable feding methods should be used when fed animals.