| 天然棕色棉查尔酮合成酶基因(GhCHS1)的克隆和实时定量表达分析 |
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| 引用本文： | 杨会娜,田新惠,李艳军,李明月,冯鸿杰,孙杰. 天然棕色棉查尔酮合成酶基因(GhCHS1)的克隆和实时定量表达分析[J]. 棉花学报, 2010, 22(1): 42-48 |
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| 作者姓名： | 杨会娜 田新惠 李艳军 李明月 冯鸿杰 孙杰 |
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| 作者单位： | 石河子大学农学院/新疆兵团绿洲生态农业重点实验室,石河子,832003 |
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| 基金项目： | 国家973前期专项(2007CB116210);;兵团科技局基础研究项目(2006JC02) |
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| 摘 要： | 根据植物查尔酮合成酶基因保守区序列设计引物,以发育18d的天然棕色棉纤维为材料,用RT-PCR结合RACE技术分离得到了一个查尔酮合成酶基因的全长cDNA(GenBank登录号:EU921263),将该基因命名为GhCHS1。实时荧光定量PCR检测显示,GhCHS1基因在棕色棉纤维细胞中优先表达,且在棕色棉纤维中的表达量远高于其近等基因系白色棉,但是该基因在绿色棉中几乎检测不到。这些试验结果暗示,该基因可能在棕色棉纤维色素形成中发挥重要作用。
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| 关 键 词： | 查尔酮合成酶 天然彩色棉 实时定量RT-PCR |
Cloning and Quantitative Analysis by Real-Time RT-PCR of a Chalcones Synthase Gene(GhCHS1)in the Natural Brown Cotton Fibers |
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| YANG Hui-na,TIAN Xin-hui,LI Yan-jun,LI Ming-yue,FENG Hong-jie,SUN Jie. Cloning and Quantitative Analysis by Real-Time RT-PCR of a Chalcones Synthase Gene(GhCHS1)in the Natural Brown Cotton Fibers[J]. Cotton Science, 2010, 22(1): 42-48 |
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| Authors: | YANG Hui-na TIAN Xin-hui LI Yan-jun LI Ming-yue FENG Hong-jie SUN Jie |
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| Affiliation: | College of Agriculture/The Key Oasis Eco-agriculture Laboratory of Xinjiang Production and Construction Group/a>;Shihezi University/a>;Shihezi/a>;Xinjiang 832003/a>;China |
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| Abstract: | A full-length cDNA encoding CHS was cloned from brown cotton fiber of 18 days post anthesis by RT-PCR and rapid amplification of cDNA ends (RACE) using specific primers based on the highly conserved sequences of plant CHS that had already known. Blast search revealed that it is a new gene,and was named as GhCHS1 (GenBank accession:EU921263). The temporal and spatial expression patterns of GhCHS1 gene were examined by the real-time quantitative PCR with the specific primers. The expression analysis indicated... |
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| Keywords: | chalcones synthase natural colored cotton real-time quantitative PCR |
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