| 白三叶草种质资源同工酶的遗传多样性分析 |
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| 引用本文： | 何俊,周康,张莉,何胜江.白三叶草种质资源同工酶的遗传多样性分析[J].贵州农业科学,2010,38(3). |
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| 作者姓名： | 何俊 周康 张莉 何胜江 |
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| 作者单位： | 1. 贵阳市农产品质量安全监督检验测试中心,贵州,贵阳,550008
2. 贵州大学,贵州,贵阳,550025 |
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| 摘 要： | 为了给白三叶草的选育和资源利用提供理论依据,采用聚丙烯酰胺凝胶电泳的方法,对来自不同地区的16份白三叶草(Trifoliumrepens L.)种质资源进行酯酶(EST)和过氧化物酶(POD)同工酶检测,并对检测到的5个等位酶位点的图谱进行分析,再根据各种质资源间遗传相似度及聚类结果进行分析。结果表明:白三叶草各种质资源间有较高的遗传多样性;16份白三叶草种质资源在相似系数为0.71的水平上可以聚为三类,大部分地理区域临近的种质材料首先聚在一起。说明他们有较近的亲缘关系。
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| 关 键 词： | 白三叶 同工酶 酯酶（EST） 过氧化物酶（POD） 遗传多样性 聚类分析 |
Genetic Adersity of Trifolium repens Germplasm Resources Based on Isozyme Analysis |
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| HE Jun,ZHOU Kang,ZHANG Li,HE Sheng-jiang.Genetic Adersity of Trifolium repens Germplasm Resources Based on Isozyme Analysis[J].Guizhou Agricultural Sciences,2010,38(3). |
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| Authors: | HE Jun ZHOU Kang ZHANG Li HE Sheng-jiang |
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| Institution: | 1.Guiyang Determination and Testing Center for Quality Safety of Agricultural Products;Guiyang;Guizhou 550008;2.Guizhou University;Guizhou 550025;China |
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| Abstract: | The esterase(EST) isozyme and peroxidase(POD) isozyme of 16 Trifolium repens germplasm resources from different regions were determined by polyarcylamide gel electrophoresis and the locus chart of 5 determined allelic enzymes were analyzed to provide a theoretical basis for breeding and resource utilization of Trifolium repens. The results showed that there was great genetic adversity among different germplasm resources. The results from the genetic similarity and clustering analysis among different germpla... |
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