| 木薯苹果酸脱氢酶基因克隆和表达分析 |
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| 引用本文： | 尹奇,仝征,贺庭琪,王力敏,黄启星,郭运玲,孔华,王旭初,郭安平.木薯苹果酸脱氢酶基因克隆和表达分析[J].热带作物学报,2013,34(6):1082-1089. |
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| 作者姓名： | 尹奇 仝征 贺庭琪 王力敏 黄启星 郭运玲 孔华 王旭初 郭安平 |
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| 作者单位： | 1. 海南大学农学院,海南海口 570228;中国热带农业科学院热带生物技术研究所,海南海口 571101
2. 中国热带农业科学院热带生物技术研究所,海南海口,571101 |
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| 基金项目： | 国家重点基础研究发展973计划（No. 2010CB126605）；中国热带农业科学院热带生物技术研究所基本科研业务费专项（No. ITBB110101，No. ITBB110102）；海南省自然科学基金（No. 310080）。 |
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| 摘 要： | 为从木薯块根中获得苹果酸脱氢酶(MDH)基因,并研究其在转录水平的表达变化规律,利用RACE技术从木薯“华南8号”块根中克隆得到了苹果酸脱氢酶基因,其cDNA全长1 175 bp,包含999 bp的开放阅读框,共编码332个氨基酸.从木薯“华南8号”中获得的MDH氨基酸序列,与其它物种的该序列相似度达84％～94％,包含细胞质苹果酸脱氢酶中高度保守的NAD结合基元“TGAAGQI”和催化基元“IWGNH”.木薯MDH基因与块根淀粉合成相关,在块根发育前期表达量较低,膨大期表达量较高,膨大后期表达量逐渐降低.
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| 关 键 词： | 木薯 块根 苹果酸脱氢酶 淀粉合成 |
Cloning and Expression Analysis of Malate Dehydrogenase Gene from Cassava |
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| YIN Qi,TONG Zheng,HE Tingqi,WANG Limin,HUANG Qixing,GUO Yunling,KONG Hu,WANG Xuchu and GUO Anping.Cloning and Expression Analysis of Malate Dehydrogenase Gene from Cassava[J].Chinese Journal of Tropical Crops,2013,34(6):1082-1089. |
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| Authors: | YIN Qi TONG Zheng HE Tingqi WANG Limin HUANG Qixing GUO Yunling KONG Hu WANG Xuchu and GUO Anping |
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| Institution: | 1 College of Agriculture, Hainan University 2 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of
Tropical Agricultural Sciences;1 College of Agriculture, Hainan University 2 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of
Tropical Agricultural Sciences;Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;1 College of Agriculture, Hainan University 2 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of
Tropical Agricultural Sciences;Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;1 College of Agriculture, Hainan University 2 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of
Tropical Agricultural Sciences;Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences |
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| Abstract: | A 1 175 bp cDNA sequence of MDH with a 999 bp open reading frame, encoding a protein with 332 amino acids, was obtained from cassava SC8 roots by RACE(rapid-amplification of cDNA ends)to study the expressional pattern of malate dehydrogenase(MDH)mRNA. The MDH shared 84%~94% of amino acid sequence identities with MDH from other species, and contained a typical NAD+ binding motif(TGAAGQI)and a catalytic motif(IWGNH), suggesting it was a cytosolic malate dehydrogenase(cyMDH)gene. MDH was perhaps involved in the process of starch accumulation in cassava, the expression of cassava MDH was up regulated during storage root thinckening. |
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| Keywords: | Manihot esculenta Crantz Tuberous roots Malate dehydrogenase Starch metabolism |
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