Electrophoretic characterization of North American potato cultivars

A total of 112 named cultivars and advanced breeding lines were assayed through horizontal starch electrophoresis to test the utility of isozyme loci as a means to objectively identify potato cultivars. Both leaf and tuber tissue were sampled in thirteen enzyme systems. Two buffer systems (Histidine-citrate, pH 5.7 and Lithium-borate, pH 8.3) were used to resolve 16 isozyme loci, of which 14 were scorable without progeny testing. A total of 43 scorable allozymes were detected. All cultivars or advanced breeding lines that were the result of hybridization were discriminated by their sum electrophoretic pattern, whereas, sports (i.e., Russet Burbank vs. Burbank) or line selections (i.e., Norgold Russet “M1” vs. “M2”) have patterns that are identical to the original mother clone. The allelic diversity within and among cultivars indicate that horizontal starch electrophoresis offers an objective means to discriminate sexually-derived potato cultivars.