Occurrence of the A2 mating type ofPhytophthora infestans in potato fields in the United States and Canada

Ten isolates ofP. infestans collected from blighted potato fields in the U.S. and Canada during the years 1983–1989 were examined for mating type on lima bean, oatmeal, and rye grain media. Two of the ten isolates produced oospores in less than 15 days when cultured in the presence of known A¹ mating types from the USA, Mexico, and Europe, indicating they are A² mating types. When the two A² isolates (one from Pennsylvania and one from Vancouver, B.C.) were cultured singly or with known A² isolates, no oospores formed after 6 weeks of culture. Pathogenicity tests showed that both isolates produced typical late blight symptoms on potato foliage and stems, and there appeared to be no difference in virulence between these two A² and the eight A¹ isolates. Inoculations with a mixture of A¹ + A² sporangia also produced oospores in host tissues. This is the first report of the presence of the A² mating type ofP. infestans in the U.S. and Canada.