Detection ofErwinia carotovora var.atroseptica in potato tubers with immunofluorescence following induction of decay |
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| Authors: | H. Vruggink S. H. De Boer |
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| Affiliation: | (1) Institute of Phytopathological Research (IPO), Wageningen, the Netherlands;(2) Present address: Department of Plant Science, University of British Columbia, V6T 1W5 Vancouver, B. ., Canada |
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| Abstract: | Summary The simplest method of inducing tubers to decay in order to detect contamination by the blackleg organism,Erwinia carotovora var.atroseptica, was to wound them and place them in a mist chamber for four days. Incubating the decayed tubers in air for a further two days improved detection of the blackleg organism by immunofluorescence, which was a more sensitive test than the double diffusion method. |
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| Keywords: | blackleg soft rot double diffusion wounding water infiltration oxygen coneentration |
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