基质和植物生长调节剂对美国流苏硬枝扦插生根的影响

以美国流苏Chionanthus virginicus的硬枝为材料，从基质和植物生长调节剂（种类、质量浓度及处理时间）等方面研究了各因素对插穗生根的影响，同时测定了插穗的营养物质质量分数变化。结果表明:基质种类对插穗各生根指标影响显著。其中V（珍珠岩）:V（蛭石）=7:3的基质中，其生根率和根系效果指数最高，分别为43.33%和7.64，其他生根指标达最佳水平，而河沙的插穗生根效果最差。正交试验中，3因素对生根率和根系效果指数的影响程度从大到小依次为:植物生长调节剂种类、处理时间、质量浓度，且均达极显著水平。其中，500 mg·L-1的吲哚丁酸（IBA）浸泡60 min的生根率和根系效果指数最高，分别为43.33%和6.65。可见，使用500 mg·L-1的IBA浸泡插穗60 min，扦插于V（珍珠岩）:V（蛭石）=7:3基质中，美国流苏生根效果最佳。扦插生根过程中，插穗内部的可溶性糖、淀粉和可溶性蛋白质质量分数大致为先下降后上升的趋势，在愈伤形成期和不定根诱导期下降，不定根形成后开始上升。IBA处理加速了插穗内部营养物质质量分数的变化，缩短了生根周期，有利于插穗生根。

Rooting of Chionanthus virginicus hardwood cuttings with media and plant growth regulators

In order to seek a better combination of rooting and understand the physiological changes during rooting. The effects on root cuttings from the hardwood of the fringe tree (Chionanthus virginicus) were studied in terms of medium, as well as type, concentration, and treatment time of plant growth regulators using an orthogonal experiment. Also, changes in nutrient content were measured. Results showed that the mediums had a strong influence on rooting indexes of the cuttings(P < 0.05). The perlite:vermiculite 7:3 (volume ratio) had the highest rooting rate (43.33%) and a root effect index (7.64), other rooting indicators of perlite:vermiculite 7:3 reached the best level. The rooting effect of cuttings in river sand was the worst. In the orthogonal experiment, importance of the three factors influencing the rooting rate and the root effect index were as follows:growth regulator type > treatment time > concentration with all reaching highly significant levels. Rooting rate (43.33%) and root effect index (6.65) were highest with cuttings soaked in 500 mg·L-1 Indolo Butyric Acid (IBA) for 60 min. In the rooting process, the content of soluble sugar, starch, and soluble protein in the cuttings first decreased (during the callus formation period and adventitious root induction period) and then increased (after adventitious root formation). Overall, C. virginicus is a difficult-to-root tree species, the IBA treatment of 500 mg·L-1 for 60 min and soaking the cutting in perlite:vermiculite 7:3, and the rooting effect was the best. The IBA treatment accelerated changes in nutrient content for the cuttings, shortened the rooting cycle, and facilitated rooting of the cuttings.