葱兰夜蛾幼虫肠道细菌群落结构与多样性分析

为明确葱兰夜蛾Brithys crini(Fabricius,1775)幼虫肠道细菌的群落结构和多样性,利用IonS5TMXL高通量测序平台对肠道细菌16S rDNA基因V3-V4变异区进行测序,应用Cutadapt、Uparse等软件整理、统计样品序列数和OUT数,分析肠道细菌的物种组成、丰度和多样性。4个虫体肠道样本测序拼接后,共得273 453条序列和在97%相似度下平均每个样本可聚类为512个OTU数目,总共注释到23门57纲86目168科347属,优势菌群为变形菌门Proteobacteria和厚壁菌门Firmicute。葱兰夜蛾肠道细菌Simpson指数、Shannon指数和Chao1指数分别为0.93~0.98,5.67~6.82和439.57~584.12。结果表明葱兰夜蛾幼虫肠道细菌多样性比较丰富,个体间微生物群落结构和多样性有差异。

Analysis of Intestinal Community Structure and Diversity of Larvae of Brithys crini

To clarify the bacteria community structure and diversity in the intestine of Brithys crini(Fabricius, 1775) larvae, the V3-V4 regions of the 16 S rDNA genes of intestinal bacteria in larvae of Brithys crini were sequenced by IonS5 TMXL techniques. The numbers of sequences and operational taxonomic units(OTUs), species composition, abundance and diversity of intestinal bacteria were analyzed using Cutadapt and Uparse softwares. A total of 273 453 reads were obtained from the four individuals. Under 97% similarity, each sample could be clustered into 512 OTUs, and annotated into 23 phyla, 57 classes, 86 orders, 168 families and 347 genera. Proteobacteria and Firmicutes were dominated. The diversity indices of Simpson, Shannon and Chao were 0.926-0.975, 5.661-6.823 and 439.565-584.118. The result indicated the high diversity of the intestinal bacteria of B. crini which differed in community structures and diversity among individuals.