38个紫薇品种亲缘关系的ISSR分析

紫薇是世界著名的夏季观赏花木之一,品种繁多、来源复杂,造成了紫薇品种在分类、鉴定上的困难。为探讨简单重复序列间扩增多态性(ISSR)分子标记方法在紫薇品种分类上的可行性,对引自美国的30个紫薇品种和中国的8个栽培品种进行ISSR亲缘关系分析。10条扩增带型清晰且重复性好的引物共获得81个位点,其中70条呈多态性,多态性百分比(PPB)为86.42%。38个紫薇品种的遗传相似性系数介于0.543 2~0.988 7,平均值为0.788 2。在遗传相似性系数0.736处,聚类结果(unweighted pair group method with arithmetic mean,UPGMA)显示,38个紫薇品种分为3个类群,在0.760处可进一步分为7个亚类群,表明ISSR标记技术能从分子水平上较为准确地体现紫薇品种间的亲缘关系。

Phylogenetic relationships among 38 cultivars of Lagerstroemia indica based on ISSR molecular markers

Lagerstroemia indica is one of the world-famous ornamental plants in summer with lots of visitors, but the sources of most L. indica cultivars are poorly known, resulting in some difficulties in cultivars classification. This study analyzed the genetic relationships among 38 cultivars of L. indica by inter-simple sequence repeat (ISSR) molecular-marked technique, and screened 10 ISSR primers to assess the genetic relationship of 30 L. indica cultivars from America and 8 L. indica cultivars from China. The result showed that a total of 81 DNA bands were amplified and 70 of which (86.42%) were polymorphic. The genetic similar coefficient of 38 cultivars ranged from 0.543 2 to 0.988 7 with the average of 0.788 2, suggesting that the genetic relationships among these 38 cultivars were relatively close. When the genetic similarity of 0.736 was used as the threshold value, 38 cultivars were divided into three major groups by unweighted pair group method with arithmetic mean (UPGMA) method cluster analysis, and these cultivars could be divided into seven groups at similarity coefficient of 0.76. ISSR technique was efficient in generating accurate information of genetic diversity and relationship of L. indica cultivars.