Genetic diversity studies of coarse and fine rice using RAPD markers |
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Authors: | Saeed Arshad Ahsan Iqbal Sehar Nawaz Nisar Ahmed |
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Institution: | (1) Department of soils, Eliseu Maciel School of Agronomy, Federal University of Pelotas, Campus UFPel, Cap?o do Le?o, RS-96001-970, Brazil;(2) Plant Genomics and Breeding Center, Eliseu Maciel School of Agronomy, Federal University of Pelotas, Campus UFPel, Cap?o do Le?o, RS-96001-970, Brazil |
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Abstract: | The availability of a genetically diverse gene pool is vitally important in varietal development. Molecular markers are being
extensively utilized to explore the genetic diversity among native and exotic germplasm. This study was designed to reveal
the genetic diversity and patterns of relationships among the 20 accessions/genotypes representative of basmati and non-basmati
rice from the existing rice gene pool using RAPD markers. Employing RAPD, 17 decamer oligonucleotide primers directed the
amplification of 116 fragments, out of which 101 were polymorphic (87.06%) while 15 fragments were monomorphic (12.93%). Similarity
coefficients had ranged from 0.47 to 0.90. The average genetic similarity was calculated 0.68 (68%). In this study, the coarse
rice genotypes showed more polymorphism (85.84%) than the fine rice genotypes (61.76%). Genotypes were clustered into 8 distinct
groups: A, B, C, D, E, F, G, and H but two genotypes, i.e., Shadab and Kangni-27 showed divergence from all the genotypes
of the groups. Therefore, these diverse genotypes may be included in future breeding programmes. |
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Keywords: | |
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