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| 利用改良CTAB法提取木薯基因组DNA | |
| 引用本文： | 闫庆祥,黄东益,李开绵,叶剑秋.利用改良CTAB法提取木薯基因组DNA[J].中国农学通报,2010,26(4):30-32. |
| 作者姓名： | 闫庆祥 黄东益 李开绵 叶剑秋 |
| 作者单位： | 1. 中国热带农业科学院热带作物品种资源研究所/农业部热带作物种质资源利用重点开放实验室,海南,儋州,571737;海南大学农学院,海南,儋州,571737 2. 海南大学农学院,海南,儋州,571737 3. 中国热带农业科学院热带作物品种资源研究所/农业部热带作物种质资源利用重点开放实验室,海南,儋州,571737 |
| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金，公益性行业科研专项项目 |
| 摘 要： | 采用改良CTAB法提取木薯基因组DNA,经琼脂糖凝胶电泳发现条带清晰,无拖尾现象。结果表明,DNA浓度在710.0～967.5ng/μL之间,OD260/OD280在1.73～1.92之间,该方法具有简便、快速、高效等特点,所提取的基因组DNA质量和纯度较高,适用于进一步的SSR、ISSR等分子标记分析。 |
| 关 键 词： | NDVI NDVI 傅利叶变换 地表覆被分类 |
| 收稿时间： | 2009/11/25 0:00:00 |
| 修稿时间： | 2009/12/2 0:00:00 |
Genomic DNA Extraction in Cassava by Modified CTAB Method |
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| Yan Qingxiang,Huangdongyi,Li Kaimian,Ye Jianqiu.Genomic DNA Extraction in Cassava by Modified CTAB Method[J].Chinese Agricultural Science Bulletin,2010,26(4):30-32. | |
| Authors: | Yan Qingxiang Huangdongyi Li Kaimian Ye Jianqiu |
| Institution: | Yan Qingxiang1,2,Huangdongyi2,Li Kaimian1,Ye Jianqiu1(1Tropical Crops Genetic Resources Institute,CATAS/Key Laboratoy of Tropical Crops Germplasm Resources Utilization,Ministry of Agriculture,Danzhou Hainan 571737,2Hainan University of Agriculture,Danzhou Hainan 571737) |
| Abstract: | In this paper,genomic DNA extraction in cassava by modified CTAB method,the extracted products had the clear band and without tailing bands which could be observed through agar gel electrophoresis.The results showed that the concentrations were between 710.0-967.5 ng/μL,and the values of OD260/OD280were between 1.73-1.92.The modified CTAB method had characteristics of simple,rapid and effective,the genomic DNA extracted had high quality and purity,it could be used for SSR,ISSR and other molecular markers. |
| Keywords: | modified CTAB cassava genomic DNA |
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