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Rapid Identification of Clavibacter michiganensis Subspecies Sepedonicus based on the Stable Low Molecular Weight RNA (LMW RNA) Profiles |
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| Authors: | José Luis Palomo Encarna Velázquez Pedro F Mateos Pablo García-Benavides Eustoquio Martínez-Molina |
| Institution: | (1) Centro Regional de Diagnóstico, Junta de Castilla y León, Salamanca, Spain;(2) Departamento de Microbiología y Genética, Lab 209, Edificio Departamental, Campus Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain;;(3) Departamento de Microbiología y Genética, Lab 209, Edificio Departamental, Campus Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain |
| Abstract: | Clavibacter michiganensis subsp. sepedonicus causes potato ring rot disease. The identification process for this bacterium is complex and long. This work demonstrates that the stable low-molecular-weight (LMW) RNA profiles allow their rapid identification. Staircase electrophoresis in polyacrylamide gels was used to analyze the LMW RNA profiles of 54 strains of C. michiganensis subsp. sepedonicus from different geographic origins. The profiles of several strains of other subspecies of C. michiganensis and other pathogens of potatoes were also analyzed. All the strains of C. michiganensis subsp. sepedonicus had the same LMW RNA profile. They had a band in class 2 of tRNA that was absent in the other subspecies of the species C. michiganensis. Also, the LMW RNA of C. michiganensis subsp. sepedonicus was different with respect to the LMW RNA profiles of other pathogens of potato. The results indicate the possible utilization of LMW RNA profiles in identification of the bacteria causing potato ring rot disease. |
| Keywords: | Clavibacter subsp sepedonicus potatoes disease RNA electrophoresis |
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