Expression of a Lysine-rich Gene in Bacillus subtilis 168 |
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| Authors: | Li Xue-lin Liu Xiao-fei Gao Xue-jun Qiao Bin Pan Hong-bao Ao Jin-xia |
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| Institution: | 1. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150000, China
2. College of Food Engineering, Harbin University of Commerce, Harbin 150076, China |
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| Abstract: | Lysine-rich protein gene (lys) was cloned from winged bean (Psophocarpus tetragonolobus (L.) DC), and cloned into prokaryotic expression vector pHT43, the recombinant plasmid pHT43/lys were constructed and then transferred into Bacillus subtilis168, upon IPTG induction, the recombinant protein was expressed, and the content of lysine was detected by HPLC. The result showed that lysine content increased by 9.85%. It was suggested that introducing lys gene into Bacillus subtilis 168 was an effective way to improve its nutrition quality. |
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| Keywords: | lysine-rich protein gene Bacillus subtilis 168 lysine content HPLC |
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