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| 硝酸银对黄瓜离体子叶培养芽再生的促进效应 | |
| 引用本文： | 曹利仙,赵鹂,唐宇力,朱诚. 硝酸银对黄瓜离体子叶培养芽再生的促进效应[J]. 甘肃农业大学学报, 2001, 36(2): 168-171 |
| 作者姓名： | 曹利仙 赵鹂 唐宇力 朱诚 |
| 作者单位： | 1. 浙江嘉兴学院医学院 2. 浙江嘉兴学院医学院, 3. 浙江省杭州市植物园, 4. 浙江大学生命科学学院, |
| 摘 要： | 以黄瓜品种“津春4号”无菌苗子叶为材料，研究了AgNO3对离体子叶芽再生频率的促进作用。结果表明，在含KT 2mg/L和NAA 0.1～0.4mg/L的MS培养基中附加AgNO3 2～6mg/L，可显著地提高离体子叶芽再生频率，其中最适培养基为：KT 2mg/L+NAA 0.3mg/L+AgNO3 2mg/L，芽再生频率达到77.2%。这种促进作用与AgNO3抑制乙烯的形成有关。 |
| 关 键 词： | 黄瓜 子叶 离体培养 芽再生 |
| 文章编号： | 1003-4315(2001)02-0168-04 |
Stimulation effect of silver nitrate on shoot regeneration in cotyledon tissue culture of cucumber |
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| Abstract: | ：Stimulation effect of silver nitrate on shoot regeneration in cotyledons of cucumber were studied. The results showed that shoot could be induced directly from cotyledons cultured on MS medium supplemented with KT 2mg/L and NAA 0.1～0.4mg/L, the shoot regeneration reached 49.5%～57.6%. Additon of AgNO3 2.0～6.0mg/L to the medium could greatly increased the frequency of shoot regeneration, but the optimun medium was Ms medium supplemented with KT 2mg/L, NAA 0.3mg/L and AgNO3 2.0mg/L, the shoot regeneration rate reached 77.2%. The stimulation effect might be related to inhibition of ethylene production in cotyledonary explants of cucumber. |
| Keywords: | ：cucumber；cotyledon；in vitro culture；shoot regeneration |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |