| 拮抗性大豆根瘤菌原生质体制备研究 |
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| 引用本文： | 王媛媛,段玉玺,陈立杰,朱晓峰.拮抗性大豆根瘤菌原生质体制备研究[J].大豆科学,2010,29(1). |
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| 作者姓名： | 王媛媛 段玉玺 陈立杰 朱晓峰 |
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| 作者单位： | 沈阳农业大学,生物科学技术学院,辽宁,沈阳110161;沈阳农业大学,植物保护学院,辽宁,沈阳110161;沈阳农业大学,植物保护学院,辽宁,沈阳110161 |
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| 基金项目： | 现代大豆产业技术体系资助项目;;沈阳农业大学青年教师科研基金资助项目(20070210) |
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| 摘 要： | 选用具有拮抗作用的根瘤菌L396,对影响该菌原生质体制备的几个重要因素进行了研究。结果表明:制备原生质体的最佳菌龄为对数生长中期,培养液中加入0.5%青霉素可以促进原生质体的形成,在溶菌酶浓度1.5 mg.mL-1、酶解温度为35℃的情况下可以较快较好的产生原生质体,同时显微观察了原生质体的释放过程。
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| 关 键 词： | 根瘤菌 原生质体 制备 影响因素 |
Protoplast Formation of Antagonistic Rhizobium |
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| WANG Yuan-yuan,DUAN Yu-xi,CHEN Li-jie,ZHU Xiao-feng.Protoplast Formation of Antagonistic Rhizobium[J].Soybean Science,2010,29(1). |
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| Authors: | WANG Yuan-yuan DUAN Yu-xi CHEN Li-jie ZHU Xiao-feng |
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| Institution: | 1.Department of Biological Science and Technology;Shenyang Agricultural University;Shenyang 110161;2.Department of Plant Protection;Liaoning;China |
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| Abstract: | Several factors affecting the protoplasts formation of Sinorhizobium.Fredii with antagonistic to SCN and root rot of soybean were studied.The growth curve was determined and the optimum cell-age for protoplasts formation was in the logarithmic growth mid-phase,the maximum yield of protoplast was accelerated when pretreated by 0.5% penicillium in the culture medium,the optimum enzyme condition was digesting at 35℃ with 1.5 mg · mL~(-1)lysozyme,the process of protoplasts release was observed using microscope.The foundation for genetic breeding of S.Fredii has been laid by using the protoplasts. |
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| Keywords: | Rhizobium Protoplast Formation Affecting factors |
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