低温生物膜中耐冷菌的分离与鉴定

[目的]对组成低温生物膜的耐冷菌进行分离纯化。[方法]从低温生物膜上分离纯化耐冷细菌，对其进行生理生化鉴定，并测定其在4℃和30℃时的生长曲线。[结果]组成低温生物膜的微生物有很多种，其中8种分别属于丛毛单胞菌属、黄杆菌属、节杆菌属、芽孢杆菌属、微球菌属、假单胞杆菌属、无色菌属和弧菌属。这8株菌中S3生长速率最高，S2生长速率最低，其他6株菌的生长速率相差不大．它们在30℃时的生长速率均高于在4℃时，且在24h时均进入对数生长期。[结论]该研究对于解决低温污水处理问题具有一定的意义。

Separation and Identification of the Psychrotolerant Bacteria in the Low Temperature Biomembrane

[Objective]The research aimed to separate and identify psychrotolerant bacteria from low temperature biomembrane.[Method]Psychrotolerant bacteria was separated and purified from low-temperature membrane, then physiological and biochemical identification of them was carried out and the growth curve of them at 4 ℃ and 30 ℃ was measured.[Result]There were many kinds of microbes which composing the low-temperature membrane,8 kinds among them belonged to Comamonas,Flavobacterium,Arthrobacter,Bacillus,Micrococcus,Pseudomona,Achromobacter,Vibrio, named S1,S2,S3,S4,S5,S6,S7,S8,respectively.S3 growed fastest and S2 growed slowest,the other 6 kinds growed with almost the same speed.All of the 8 kinds of microbes growed faster under 30 ℃ than under 4 ℃,and after 24 h they reached exponential phase.[Conclusion]This study was meaningful for the settlement of problems in sewage treatments under low temperature.