猪转移因子对犬中性粒细胞数量和活性的影响

按标准方法提取制备了猪的转移因子（transfer factor, TF），用吞噬杀伤试验MTT法检测了供试杂种牧羊犬肌肉注射猪TF后外周血中性粒细胞吞噬杀伤活性的变化。试验摸索出MTT法测定犬外周血中性粒细胞吞噬杀伤大肠杆菌的最佳条件为：中性粒细胞浓度1.3×106个/ml、大肠杆菌浓度6×105个/ml 时，大肠杆菌和中性粒细胞混合培养2 h，加入MTT后继续培养4 h。体外试验结果表明，猪TF浓度在0.052～1.56 mg/ml范围内，能够明显促进中性粒细胞吞噬杀菌作用，当猪TF浓度为1.56 mg/ml时，对中性粒细胞杀菌活性的影响最大。体内试验结果表明，注射猪TF后第2 d，外周血中性粒细胞数量最高，中性粒细胞吞噬杀菌能力最强。

Effects of Swine Transfer Factor on Number and Bactericidal Activity of Neutrophilic Granulocytes from Canines in Vitro

The requisite swine transfer factor (TF) was prepared by relative standards and was intramuscularly injected to a range of hybrid shepherd dogs to determine the number and bactericidal activity of neutrophilic granulocyte in canine peripheral blood by phagocytosis MTT. And the best condition for MTT to be applied into determining the ability of phagocytosis of neutrophilic granulocyte against Escherichia coli in canine peripheral blood were explored as 1.3×106 cells/ml of neutrophilic granulocytes and 6×105 units /ml had been mixed and incubated together for 2 hours followed by adding MTT and incubating for another 4 hours. The results of experiments in vitro showed that swine TF at 0.052～1.56 mg/ml markedly promoted the phagocytosis of neutrophilic granulocyte against E coli, with the strongest effect to the phagocytosis of neutrophilic granulocyte against E coli at 1.56 mg/ml; The number of neutrophilic granulocytes in canine peripheral blood climbed to the summit and the effect of phagocytosis of neutrophilic granulocyte against E coli was the strongest on the 2nd day after the dogs were intramuscularly injected with swine TF.