| 根癌农杆菌介导的番茄高效遗传转化体系的研究 |
| |
| 引用本文： | 梁超,王景雪,裴雁曦,杨婷. 根癌农杆菌介导的番茄高效遗传转化体系的研究[J]. 华北农学报, 2009, 24(2) |
| |
| 作者姓名： | 梁超 王景雪 裴雁曦 杨婷 |
| |
| 作者单位： | 山西大学,生命科学与技术学院,山西,太原,030006 |
| |
| 基金项目： | 山西省太原市科技项目 |
| |
| 摘 要： | 以番茄无菌苗的子叶和下胚轴为外植体,通过农杆菌介导法,对其遗传转化条件进行了优化,建立了高效番茄子叶和下胚轴农杆菌介导的遗传转化体系.结果表明:在农杆菌浸染前(浸染浓度为OD600=0.3)进行2 d的预培养浸染6 min的外植体在MS+2.0 mg/L6-BA+0.5 mg/L IAA的培养基上转化效率最高.PCR检测初步证明,NPTII基因已整合到番茄再生植株中.
|
| 关 键 词： | 农杆菌介导法 遗传转化 番茄 |
Study on Transformation Efficiency by Agrobacterium-mediated in Tomato |
| |
| LIANG Chao,WANG Jing-xue,PEI Yan-xi,YANG Ting. Study on Transformation Efficiency by Agrobacterium-mediated in Tomato[J]. Acta Agriculturae Boreali-Sinica, 2009, 24(2) |
| |
| Authors: | LIANG Chao WANG Jing-xue PEI Yan-xi YANG Ting |
| |
| Affiliation: | College of Life Science and Technology;Shanxi University;Taiyuan 030006;China |
| |
| Abstract: | Tomato(Lycopersicon esculentum Mill)cultivars,Texuan-28,Taiyuan 823 were used as the materials,and cotyledon and hypocotyls were cultured to optimize the transformation system via Agrobacterium.The results showed that the highest transformation efficiency was obtained when the explants were preconditioned on preconditioning medium for 2 d prior to infect with Agrobacterium(OD600=0.3)for 6 min growing on the culture medium of MS+2.0 mg/L 6-BA+0.5 mg/L IAA.It was proved that the target NPTII gene had been int... |
| |
| Keywords: | Agrobacterium tumefaciens Transgenic transformation Tomato |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
