| 传染性法氏囊病病毒致弱毒株的培育及其遗传稳定性检测 |
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| 引用本文： | 孙秋艳,刘红芹,朱瑞良. 传染性法氏囊病病毒致弱毒株的培育及其遗传稳定性检测[J]. 家畜生态, 2006, 0(2) |
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| 作者姓名： | 孙秋艳 刘红芹 朱瑞良 |
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| 作者单位： | 山东农业大学动物科技学院 山东泰安271018 |
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| 基金项目： | 国家自然科学基金(39893290)，江苏高校省级重点实验室开放课题(kjs02030)，山东省优秀中青年科学家科研奖励基金(03BS140)，山东省教育厅科研项目(J02H05)资助 |
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| 摘 要： | 隆化病毒株GX-IBDVE10C25Cl5为研究对象,将此毒株通过鸡胚成纤维细胞(CEF)连传25代。通过对克隆化毒株1、5、10、15、20、25代次传代毒TCID50(0.1ml)的测定及对41日龄的SPF鸡的致病性试验,结果表明克隆化毒株不同代次传代毒致病力逐代降低,从15代毒开始免疫器官指数、病理组织学变化与健康对照鸡相比差异不显著,由此证明此毒株已经被驯化为弱毒株,并且随着传代代次的增加遗传性能稳定。该弱毒株有望为控制vvIBDV的感染提供一条新的途径。
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| 关 键 词： | 超强毒IBDV 克隆化 试纸膜 TCID50 弱毒株 |
Selection and Cultivation of Attenuated Virus Strains of vvIBDV and Its Genetic Stability Examination |
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| SUN Qiu-yan,LIU Hong-qin,ZHU Rui-liang. Selection and Cultivation of Attenuated Virus Strains of vvIBDV and Its Genetic Stability Examination[J]. Ecology of Domestic Animal, 2006, 0(2) |
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| Authors: | SUN Qiu-yan LIU Hong-qin ZHU Rui-liang |
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| Affiliation: | SUN Qiu-yan,LIU Hong-qin,ZHU Rui-liang~* |
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| Abstract: | This experiment involved in the cell-cloned strains GX-IBDVE10C25Cl5 which come from GX8/99 of vvIBDV isolated in China.Let this strains passage on CEF for 25 generations.With clone-cell-passaged virus,TCID50(0.1ml)and the pathogenicity experiment on 41-day SPF chicken were tested.The result demonstrated that clone-cell-passaged virus virulence is slowly decreasing;there is little difference between the immune index,changing of pathological organs and normal chickens.This result proved that clone-cell-passaged strain has been cultivated attenuated virus.With the passage increasing,the transmissibility is stable.It is expected that this attenuated strains can offer a new way in controlling the infection of vvIBDV. |
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| Keywords: | vvIBDV passage Strip TCID50 attenuated strain |
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