重瓣大花萱草组织培养快速繁殖的研究

试验以重瓣大花萱草带生长点的茎段为试材 ,以MS与 1/ 2MS为基本培养基 ,分别添加不同浓度的 2 ,4 -D ,6 -BA ,NAA。试验结果表明 :MS 6 -BA1mg/l NAA0 1mg/l的固体培养基 ,有很好的诱导外植体产生不定芽苗的效果 ;而MS 2 ,4 -D2mg/l 6 -BA0 1mg/l的培养基能很快诱导外植体产生愈伤组织 ;愈伤组织在MS 6 -BA1mg/l的培养基上培养后 ,形成结构致密的球状体愈伤组织 ,并分化出苗 ,经继代培养后 ,形成丛状苗 ;试管苗的生根是以 1/ 2MS NAA0 5mg/l的固体培养基最为合适。

High - speed breed of tissue culture of double flower Hemerocallis fulva

The experiments were used the stem with growing point of double flower Hemerocallis fulva as test plant.The MS and 1/2 MS as culture medium were each added different concentration 2,4-D,6-BA and NAA.The results of experiments:MS added 6-BA 1mg/L and NAA 0 1mg/L solid culture medium had good induced effect to explantation growth out adventitious bud.MS added 2,4-D 2mg/L and 6-BA 0 1mg/L culture medium can induced the explantation growth callus quickly.The callus after cultured in MS added 6-BA 0 1mg/L,the compact construction sphacroplast callus was formed and differentiation,after generation cultured,the clamp seedling was formed.And 1/2 MS added NAA 0 5mg/L solid culture medium was the best medium for test tube seedling growth root.