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| 枯草芽孢杆菌固态发酵麻疯树废弃饼粕产蛋白酶初步研究 | |
| 引用本文： | 杜亚菲,吴远根,吴洁,满敬杰,郭云兰,邱树毅.枯草芽孢杆菌固态发酵麻疯树废弃饼粕产蛋白酶初步研究[J].贵州农业科学,2009,37(11). |
| 作者姓名： | 杜亚菲 吴远根 吴洁 满敬杰 郭云兰 邱树毅 |
| 作者单位： | 1. 贵州大学,贵州省发酵工程与生物制药重点实验室,贵州,贵阳,550003;贵州大学,化学与化工学院,贵州,贵阳,550003 2. 贵州大学,化学与化工学院,贵州,贵阳,550003 3. 贵州大学,贵州省发酵工程与生物制药重点实验室,贵州,贵阳,550003贵州大学,化学与化工学院,贵州,贵阳,550003 |
| 基金项目： | 教育部"春晖"计划"微生物发酵辅助提取麻疯树油脂并耦联产饲料研究"，贵州省科学技术基金"麻疯树果提油后饼粕的发酵脱毒与综合利用研究"，国家大学生创新性实验计划"固态发酵麻疯树饼粕产蛋白酶及酶学性质的研究"，贵州大学大学生创新性实验计划"固态发酵麻疯树饼粕产蛋白酶的初步研究" |
| 摘 要： | 为寻找出麻疯树制备生物柴油后废弃饼粕合理利用的有效途径,利用枯草芽孢杆菌作为发酵菌株,以提油后的麻疯树饼粕作为培养基,采用固态发酵方式进行产蛋白酶试验.结果表明:控制培养基相对湿度为150%,37℃下发酵2 d,蛋白酶产量达到3 284 U/g;向培养基中添加15%葡萄糖,则蛋白酶产量达到12 108 U/g,比对照组提高3.6倍;向培养基中添加氮源后,蛋白酶产量反而下降. |
| 关 键 词： | 麻疯树饼粕 枯草芽孢杆菌 蛋白酶 固态发酵 |
Study on Protease Production from De-oiled Jatropha curcas Seed Cakes by Bacillus subtilis in Solid-state Fermentation |
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| DU Ya-fei,WU Yuan-gen,WU Jie,MAN Jing-jie,GUO Yun-lan,QIU Shu-yi.Study on Protease Production from De-oiled Jatropha curcas Seed Cakes by Bacillus subtilis in Solid-state Fermentation[J].Guizhou Agricultural Sciences,2009,37(11). | |
| Authors: | DU Ya-fei WU Yuan-gen WU Jie MAN Jing-jie GUO Yun-lan QIU Shu-yi |
| Institution: | DU Ya-fei1,2,WU Yuan-gen1,WU Jie2,MAN Jing-jie2,GUO Yun-lan2,QIU Shu-yi1,2(1.Guizhou Key Laboratory of Fermentation Engineering and Biopharmacy,Guizhou University,Guiyang,Guizhou 550003,2.School of Chemistry and Chemical Engineering,China) |
| Abstract: | The test of protease production from de-oiled Jatropha curcas seed cake (JCSC) by Bacillus subtilis in solid-state fermentation was conducted to find the effective ways to rationally utilize de-oiled seed cakes of Jatropha curcas. The results showed that the protease yield was 3 284 U/g when 150% relative humidity of JCSC was fermented by Bacillus subtilis at 37 ℃ for 2 days. The protease yield could be up to 12 108 U/g when 15% glucose was added in JCSC and was 3.6 times higher than CK. The protease yield decreased when nitrogen source was added in JCSC. |
| Keywords: | Jatropha curcas seed cake (JCSC) Bacillus subtilis protease solid-state fermentation |
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