| SSEA-1、SSEA-3、Oct-4及hTERT在人胚胎生殖细胞中的表达 |
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| 引用本文： | 李芳,陈永珍,陈谦,朱旻,张苏. SSEA-1、SSEA-3、Oct-4及hTERT在人胚胎生殖细胞中的表达[J]. 扬州大学学报(农业与生命科学版), 2007, 28(4): 21-24 |
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| 作者姓名： | 李芳 陈永珍 陈谦 朱旻 张苏 |
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| 作者单位： | 苏州大学,医学院,江苏,苏州,215123;苏州大学,医学院,江苏,苏州,215123;苏州大学,医学院,江苏,苏州,215123;苏州大学,医学院,江苏,苏州,215123;苏州大学,医学院,江苏,苏州,215123 |
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| 基金项目： | 苏州市社会发展和三药基金资助项目(N3134507) |
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| 摘 要： | 分离5~9周人胚胎生殖腺嵴和背侧肠系膜,采用组织块法进行体外培养,免疫细胞化学染色法检测SSEA-1、SSEA-3、Oct-4及hTERT在人胚胎生殖细胞中的表达,以证实利用组织块体外培养所获得的细胞为未分化的人胚胎生殖细胞。结果表明:组织块体外培养得到的细胞或细胞集落均阳性表达SSEA-1、SSEA-3、Oct-4及hTERT,说明组织块体外培养获得的细胞为未分化的人胚胎生殖细胞。
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| 关 键 词： | 人胚胎生殖细胞 SSEA-1 SSEA-3 Oct-4 hTERT 表达 |
| 文章编号： | 1671-4652(2007)04-0021-04 |
| 修稿时间： | 2007-07-28 |
The expression of SSEA-1, SSEA-3, Oct-4 and hTERT in human embryonic germ cells |
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| LI Fang,CHEN Yong-zhen,CHEN Qian,ZHU Min,ZHANG Su. The expression of SSEA-1, SSEA-3, Oct-4 and hTERT in human embryonic germ cells[J]. Journal of Yangzhou University(Agricultural and Life Science Edition), 2007, 28(4): 21-24 |
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| Authors: | LI Fang CHEN Yong-zhen CHEN Qian ZHU Min ZHANG Su |
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| Abstract: | Human embryonic genital ridges,dorsal mesenteries(5-9 weeks postfertilization) were cultured through tissue culturing and then use the immunocytochemistrical staining to observe the expression of SSEA-1,SSEA-3,Oct-4,and hTERT on the cultivated cells to show these cells cultured through tissue culturing were undifferentiated human embryonic germ cells.Both the individual cell,and the cluster of cells which cultured in vitro through tissue culturing are SSEA-1,SSEA-3,Oct-4,and hTERT positive,so it shows that the cells we obtained through tissue culturing are undifferentiated human embryonic germ cells. |
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| Keywords: | human embryonic germ cells SSEA-1 SSEA-3 Oct-4 hTERT expression |
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