拟南芥DNA_J结构域蛋白的生物信息学分析及其亚细胞定位验证

对拟南芥RSS1蛋白进行了一系列生物信息学分析,结果表明:该蛋白是一C端含有DNA_J结构域的辅助蛋白,其N端含有受体蛋白复合物AP-2结合区DPF/W和FxDxF区,中间区域含有可信度较低的丝氨酸富集区和谷氨酸富集区.该蛋白可能具有ATP结合活性、丝氨酸/苏氨酸激酶活性和磷酸酶活性,可调控细胞循环和蛋白的磷酸化和去磷酸化作用.利用洋葱表皮瞬时表达系统验证了RSS1-GFP融合蛋白定位于细胞核中,为进一步研究该蛋白的分子和生物学功能奠定了良好基础.

Bioinformatics and subcellular localization analysis of a protein containing the DNA_J domain of Arabidopsis thaliana

A series of bioinformatics analysis of RSS1 protein of Arabidopsis thaliana was analyzed.The result showed that RSS1 had a J-domain,resembling clathrin-uncoating factor auxilin at its C terminus.It also contained an Asp-Pro-Phe(DPF) adaptor protein-binding motif and FxDxF motif at the N terminus,which was known to bind to the appendage domain of α or β subunits of an adaptor protein complex AP-2.Moreover,there were a serine-rich region and a glutamic acid-rich region in RSS1.The bioinformatics data suggested that RSS1 might have ATP-binding activity,serine/thrine kinase or phosphatase activity and it might regulate cell cycle or phosphorylation or dephosphorylation of proteins.Subcellular localization by instant expression of RSS1-GFP fusion protein on onion epidermis showed that RSS1 was localized in nucleolus,which would help to further characterize molecular or biological functions of the protein.