生物素—亲和素法检测雏鸡实验性新城疫的研究

采用生物素—亲和素法检测实验鸡雏35只。结果,接毒12h,在法氏囊滤泡的髓质、胸腺小叶的髓质、脾白髓的脾小结、盲肠扁桃体的淋巴小结及弥散淋巴组织内首先出现核浓缩,胞浆呈棕色的阳性淋巴细胞。接毒1～3d,在骨髓血窦外区、法氏囊滤泡、胸腺小叶、脾白髓、盲肠扁桃体和弥散淋巴组织内出现核浓缩、碎裂,泡浆呈棕色的阳性淋巴细胞、阳性巨噬细胞和阳性网状细胞。接毒4～5d死亡病例的这些免疫器官原有结构破坏,形成蜂窝状空泡结构,空泡含有核碎屑和棕色微粒。肝、肾、肺的微血管充满红细胞凝集,其胞膜显现深棕色微粒。根据这些变化可建立雏鸡新城疫诊断。

Study of Experimental Newcastle Disease in Chickens Detected by BA Method

35 experimental chickens were detected by biotin-avidin(BA) method. The lymphocytes showing pyknotic nuclei and brown cytoplasmswere first found in the medulla of the bursal follicles and the thymiclobules,the splenic corpuscles of the white pulp,the lymphatic nodulesof the cecal tonsils and the diffuse lymphoid tissue at 12 hours afterinfection.The lymphocytes,macrophages and reticular cells appearingshrunk, broken nuclei and brown cytoplasms were observed in theperipheral zone of the sinusoids of the bone marrow,the bursalfollicles,the thymic lobules,the splenic white pulp,the cecal tonsilsand the diffuse lymphoid tissue at 1-3 days after infection. Thestructure of the immune system des troyed and formed honeycombedvacuoles containing nuclear debris and brown granules in died cases of4-5 days after infection. The micrangii of liver,kidney and lugs werefilled with agglutinative erythrocytes that show brown grameles ontheir cytoplasmic membranae.By these changes the diagnosis of ND inchickens can be confirmed.