| 猪繁殖与呼吸综合征病毒GP5、M蛋白基因的克隆及其基因疫苗构建 |
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| 引用本文： | 马德慧,薛江东,刘锴,姚春雨,杨明霞.猪繁殖与呼吸综合征病毒GP5、M蛋白基因的克隆及其基因疫苗构建[J].中国兽医学报,2010,30(2). |
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| 作者姓名： | 马德慧 薛江东 刘锴 姚春雨 杨明霞 |
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| 作者单位： | 内蒙古民族大学,动物科技学院,内蒙古,通辽,028000 |
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| 基金项目： | 内蒙古自治区人才基金资助项目 |
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| 摘 要： | 参照GenBank中猪繁殖与呼吸综合征病毒(PRRSV)美洲型代表株VR2332 GP5和M蛋白基因序列,设计并合成2对引物,用RT-PCR方法分别扩增出PRRSV野毒株GP5和M蛋白基因603,525bp片段,并将其分别克隆到pMD18-T载体。测序正确后,将GP5和M蛋白基因分别克隆到真核表达载体pEGFP-C1上,成功构建基因疫苗表达载体pEGP5-C1和pEM-C1。小鼠免疫试验证实,这些基因疫苗质粒可以诱导小鼠产生特异性抗体,并在二免后1周开始检测到特异性淋巴细胞增殖反应。
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| 关 键 词： | 猪繁殖与呼吸综合征病毒 GP5蛋白基因 M蛋白基因 基因疫苗 |
Cloning of GP5 and M proteins gene from porcine reproductive and respiratory syndrome virus and studies on its DNA vaccines |
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| MA De-hui,XUE Jiang-dong,LIU Kai,YAO Chun-yu,YANG Ming-xia.Cloning of GP5 and M proteins gene from porcine reproductive and respiratory syndrome virus and studies on its DNA vaccines[J].Chinese Journal of Veterinary Science,2010,30(2). |
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| Authors: | MA De-hui XUE Jiang-dong LIU Kai YAO Chun-yu YANG Ming-xia |
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| Institution: | MA De-hui,XUE Jiang-dong,LIU Kai,YAO Chun-yu,YANG Ming-xia (College of Animal Science , Technology,Inner Mongolla University for Nationality,Tongliao,Inner Mongolla 028000,China) |
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| Abstract: | Based on GenBank database of porcine reproductive and respiratory syndrome virus (PRRSV) American strain VR2332 (Accession:EF536003),two pairs of primer were designed and synthesized.GP5 and M proteins gene of PRRSV street strain were amplified by RT-PCR and cloned into pMD18-T vector.The sequencing results indicated that GP5 protein gene consisted of 603 nucleotides and M protein gene was 525.GP5 and M proteins gene were subcloned into eukaryotic expression vector pEGFP-C1,to construct the expression plasm... |
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| Keywords: | porcine reproductive and respiratory syndrome virus GP5 protein gene M protein gene DNA vaccine |
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