大豆脂肪氧化酶缺失体检测方法研究

薄层等电聚焦电泳技术(IEF)具有准确、快速、分辨率高的特点.作者对IEF-PAGE电泳制胶及酶染技术进行改进,应用于大豆脂肪氧化酶同工酶类型鉴定,获得很好效果,大大降低实验成本,为此,作者研究制定了一套准确、简便的大豆脂肪氧化酶缺失体检测方法.

THE IDENTIFI ATION TECHNIQUE OF SOYBEAN LIPOXYGENASE

The grass-bean flavor of soybean food stuff is related to lipoxygenase(Lox ). In processing, the physical or chemical methods are used to remove the undesirable flavor and odor of soybean food stuff, but these methods often lead to some insolubility of soybean proteins, and decrease the quality of protein. So it is necessary and urgent to select and develop mutant soybean by genetic study . Lipoxygenase lacking mutants (triple - null or double - null ) soybean could improve nutritional quality and functionality of soybean products . There are four known electrophoresis types of soybean seed lipoxygenase (Lox ) . The Lox isozymes have their own biochemistry characteristic . IEF-PAGE helps us to identity Lox isozyme type and mutants . We modified the techniques about making gel and staining the gel for improving the resolution and reducing the experiment cost .The GelBond PAG sheet was replaced by normal glass plate .Use suitable enzyme staining solution . The tests attained remarkably results and reduced cost . Thus ,a technique for identifying Lox lacking mutants was developed ,that was rapid and more accuracy .