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Quantitative correlation of parasitological and serological techniques for the diagnosis of Trypanosoma congolense infection in cattle |
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| Authors: | F. Jongejan C. J. P. G. Ooijen D. Zivkovic A. A. H. M. ter Huurne |
| Affiliation: | 1. UNDP/FAO, Animal Disease Control Project , P.O. Box 30563, Lusaka, Zambia;2. Department of Tropical Veterinary Medicine and Protozoology, Faculty of Veterinary Medicine , State University of Utrecht , P.O. Box 80.172, Utrecht, 3508 TD, The Netherlands;3. Animal Disease Control Project , UNDP/FAO , P.O. Box 30563, Lusaka, Zambia;4. International Atomic Energy Agency , Vienna, Austria;5. Department of Tropical Veterinary Medicine and Protozoology , State University of Utrecht , P.O. Box 80.172, Utrecht, 3508 TD, The Netherlands;6. Department of Experimental Zoology , State University of Utrecht , Padualaan 8, Utrecht, 3584 CH, The Netherlands;7. District Veterinary Officer, P.O. Box 910034, Mongu, Western Province, Zambia |
| Abstract: | Summary A comparison was made between serological and parasitological techniques for the diagnosis of bovine trypanosomiasis in Zambia. Overall sero‐prevalence rates as determined by IFAT and ELISA were respectively 2.7‐fold and 2.9‐fold greater then the percentage of samples found positive with the dark ground/phase contrast buffy coat technique (DG). The results obtained by the two serological techniques were found to be closely correlated (94.2% agreement) and titres obtained by ELISA tended to be slightly higher than those obtained by IFAT. Linear regression analysis of the results obtained by the IFAT and DG techniques revealed a highly significant correlation. This finding would permit the use of only one of the techniques in an epidemiological survey and to extrapolate the results from the regression line. |
| Keywords: | pluripotent stem cells embryonic stem cells germ line stem cells artificial gametes infertility reproduction |