| 黄瓜根结RDR酶基因的分离与表达分析 |
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| 引用本文： | 张婧妹,茆振川,刘峰,陈国华,杨宇红,冯东昕,谢丙炎.黄瓜根结RDR酶基因的分离与表达分析[J].园艺学报,2011,38(10):1911-1920. |
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| 作者姓名： | 张婧妹 茆振川 刘峰 陈国华 杨宇红 冯东昕 谢丙炎 |
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| 作者单位： | 中国农业科学院蔬菜花卉研究所,农业部蔬菜种质遗传改良重点实验室,北京100081 |
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| 基金项目： | 国家重点基础发展计划项目(2009CB119000); 公益性行业(农业)科研专项(201103018); 国家自然科学基金重点项目(31030057);国家自然科学基金项目(30971905); 国家大宗蔬菜产业技术体系项目 |
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| 摘 要： | 基于黄瓜（Cucumis sativus L.）自交系9930全基因组序列，采用RT-PCR的方法，分离出根结形成过程中表达的RDR基因，共获得了5个CsRDR基因：CsRDR1a、CsRDR1b、CsRDR1c、CsRDR2和CsRDR6（HQ738485 ~ HQ738489）。qPCR定量分析表明，5个基因在接种根结线虫后的不同时期表达量都有不同程度的增加。CsRDR1a、CsRDR1b、CsRDR2和CsRDR6在接种线虫早期（6 h）相对表达量明显增加；而CsRDR1c在接种36 h时相对表达量达到最高,从而表明CsRDR基因与黄瓜根结的发育相关。
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| 关 键 词： | 黄瓜 根结 RDR 分离 表达 |
Isolation and Expression Analysis of CsRDRs in Root-knot of Cucumis sativus |
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| ZHANG Jing-mei,MAO Zhen-chuan,LIU Feng,CHEN Guo-hua,YANG Yu-hong,FENG Dong-xin, XIE Bing-yan.Isolation and Expression Analysis of CsRDRs in Root-knot of Cucumis sativus[J].Acta Horticulturae Sinica,2011,38(10):1911-1920. |
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| Authors: | ZHANG Jing-mei MAO Zhen-chuan LIU Feng CHEN Guo-hua YANG Yu-hong FENG Dong-xin XIE Bing-yan |
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| Institution: | ZHANG Jing-mei,MAO Zhen-chuan,LIU Feng,CHEN Guo-hua,YANG Yu-hong,FENG Dong-xin,and XIE Bing-yan(Institute of Vegetables and Flowers,Key Laboratory of the Ministry of Agriculture on Genetic Improvement for Vegetable Germplasm,Chinese Academy of Agricultural Sciences,Beijing 100081,China) |
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| Abstract: | To indentify the RDR genes and their expression characteristics involved in the root-knot development of cucumber(Cucumis sativus L.),RT-PCR and qPCR approaches were used in this study.Five CsRDR genes were isolated from root-knot of cucumber via RT-PCR,including CsRDR1a,CsRDR1b,CsRDR1c,CsRDR2 and CsRDR6(GenBank No.HQ738485-HQ738489).qPCR analysis showed that the expression of five CsRDR genes were increased at different levels in different stages upon Meloidogyne incognita infection.CsRDR1a,CsRDR1b,CsRDR2 ... |
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| Keywords: | Cucumis sativus root-knot RDR isolation expression |
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