| 胡椒属植物DNA条形码初步研究 |
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| 引用本文： | 郝朝运,邬华松,范睿,杨建峰,吴刚,马腾飞,秦晓威. 胡椒属植物DNA条形码初步研究[J]. 热带作物学报, 2013, 34(5): 870-874 |
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| 作者姓名： | 郝朝运 邬华松 范睿 杨建峰 吴刚 马腾飞 秦晓威 |
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| 作者单位： | 1 中国热带农业科学院香料饮料研究所2 农业部香辛饮料作物遗传资源利用重点实验室3 海南省热带香辛饮料作物遗传改良与品质调控重点实验室;1 中国热带农业科学院香料饮料研究所2 农业部香辛饮料作物遗传资源利用重点实验室3 海南省热带香辛饮料作物遗传改良与品质调控重点实验室;1 中国热带农业科学院香料饮料研究所2 农业部香辛饮料作物遗传资源利用重点实验室3 海南省热带香辛饮料作物遗传改良与品质调控重点实验室;1 中国热带农业科学院香料饮料研究所2 农业部香辛饮料作物遗传资源利用重点实验室3 海南省热带香辛饮料作物遗传改良与品质调控重点实验室;1 中国热带农业科学院香料饮料研究所2 农业部香辛饮料作物遗传资源利用重点实验室3 海南省热带香辛饮料作物遗传改良与品质调控重点实验室;云南大学生命科学学院;1 中国热带农业科学院香料饮料研究所2 农业部香辛饮料作物遗传资源利用重点实验室3 海南省热带香辛饮料作物遗传改良与品质调控重点实验室 |
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| 基金项目： | 国家自然科学基金项目（No. 31201263）;农业部南亚热作专项（No. 12RZZY-13）。 |
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| 摘 要： | 为筛选胡椒属DNA条形码最佳片段,研究了ITS、rbcL、psbJ-petA和matK基因片段的有效使用性、种内种间变异和barcoding gap,并评估了序列鉴定效率。结果显示：ITS和matK的barcoding gap图相对较好,matK物种水平鉴定成功率高,ITS种间变异较大,而其他2个候选序列不能进行有效鉴定。为此,推荐matK和ITS作为胡椒属植物潜在的DNA条形码序列,并依此探索建立该属的DNA条形码鉴定方法。
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| 关 键 词： | 胡椒属 DNA条形码 ITS psbJ-petA matK rbcL |
DNA Barcoding in Genus Piper |
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| HAO Chaoyun,WU Huasong,FAN Rui,YANG Jianfeng,WU Gang,MA Tengfei and QIN Xiaowei. DNA Barcoding in Genus Piper[J]. Chinese Journal of Tropical Crops, 2013, 34(5): 870-874 |
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| Authors: | HAO Chaoyun WU Huasong FAN Rui YANG Jianfeng WU Gang MA Tengfei QIN Xiaowei |
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| Affiliation: | 1 Spice and Beverage Research Institute, CATAS2 Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture3 Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulatioin for Tropical Spice and;1 Spice and Beverage Research Institute, CATAS2 Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture3 Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulatioin for Tropical Spice and;1 Spice and Beverage Research Institute, CATAS2 Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture3 Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulatioin for Tropical Spice and;1 Spice and Beverage Research Institute, CATAS2 Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture3 Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulatioin for Tropical Spice and;1 Spice and Beverage Research Institute, CATAS2 Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture3 Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulatioin for Tropical Spice and;School of Life Sciences, Yunnan University;1 Spice and Beverage Research Institute, CATAS2 Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture3 Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulatioin for Tropical Spice and |
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| Abstract: | In order to screen right DNA regions in Piper, four candidate DNA barcodes with three(rbcL, psbJ-petA, matk)from chloroplast genome and one(ITS)from the nuclear genome, were evaluated in view of 35 self-testing accessions and 282 ones from NCBI. Capability of the four candidate DNA barcodes was evaluated by effectiveness, intra-and inter-specific divergence and barcoding gap analysis, and the identification efficiency was also assessed using Neighbour-Joining method. The results showed that ITS and matK candidate barcodes had clear barcoding gap. At the same time, matK had high species identification reliability, and ITS had high significant divergence at species level. The other two candidate barcodes had no clear barcoding gap. matK and ITS might be the potential DNA barcoding for the identification of Piper plants, thus making the establishment of new identification methods for this genus possible. |
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| Keywords: | Piper DNA barcoding ITS rbcL psbJ-petA matK |
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