不同培养基对紫薇试管苗的诱导·增殖·生根的影响

目的]提高紫薇的繁殖速度,建立紫薇的快速繁殖体系。方法]以紫薇种子为外植体,在MS基本培养基中添加不同浓度的6-BA、NAA、KT,研究不同培养基对紫薇试管苗的诱导、增殖和生根的影响。结果]含有一定浓度激素的培养基中的种子的发芽率明显高于不含任何激素的培养基,紫薇试管苗的诱导培养基为:MS+6-BA1.0mg/L+NAA0.01mg/L。随着6-BA、KT、NAA浓度的变化,茎段的出芽率、芽平均长度及生长情况都有较明显的变化,适宜增殖培养基是MS+6-BA2.0mg/L+KT1.0mg/L+NAA0.01~0.05mg/L。适宜生根培养基为:MS+NAA0.5mg/L,试管苗生长健壮,根系粗壮,生根数较多。结论]6-BA、KT、NAA等激素的浓度对紫薇丛生芽的形成和生根有较大的影响。

Effect of Different Media on Induction,Proliferation and Rooting of Lagerstroemia indica Tuhe Seedlings

Objective] The propose of the study was to increase the propagation rate of Lagerstroemia indica and establish the its rapid propagation system. Method] With L. indica seeds as the explants, 6-BA, NAA and KT with different concn. were added into MS medium to study the effect of different media on induction, proliferation and rooting of L. indica tube seedlings. Result] The germination rate of L. indica seeds on medium with the hormone at some concn. was obviously higher than that in medium without the hormone. The medium for inducing the L. indica tube seedlings was MS+6-BA 1.0 mg/L+NAA 0.01 mg/L. The budding rate, mean length and growth state of bud varied obviously with the change of 6-BA, NAA and KT concn. The optimum medium for proliferation was MS+6-BA 2.0 mg/L+KT 1.0 mg/L+NAA 0.01～0.05 mg/L MS+NAA 0.5 mg/L. The optimum medium for rooting was MS+NAA 0.5 mg/L, on which, the tube seedlings grown strongly with more and thick roots. Conclusion] The concn. of hormone such as 6-BA, NAA and KT had great effect on forming and rooting of L. indica clustered shoots.