| 类胡萝卜素合成酶基因LycB导入番茄的研究 |
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| 引用本文： | 抗艳红,季静,吴东,王罡.类胡萝卜素合成酶基因LycB导入番茄的研究[J].中国蔬菜,2006,1(5):11-13. |
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| 作者姓名： | 抗艳红 季静 吴东 王罡 |
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| 作者单位： | 河北北方学院农业科学系; 天津大学农业与生物工程学院; 天津大学防化指挥工程学院; 300072 |
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| 基金项目： | 国家植物转基因技术研究所开发与中试基地基金 |
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| 摘 要： | 以4个番茄品种的子叶为受体,以LycB为目的基因,应用根癌农杆菌介导法对番茄进行了遗传转化,经愈伤诱导、芽诱导、生根培养和潮霉素选择培养,获得的抗性植株经PCR和PCR-Southern分析鉴定,初步证明外源基因LycB已整合到番茄的基因组中。
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| 关 键 词： | LycB基因 番茄 子叶 转化 |
| 收稿时间： | 2005-08-05 |
| 修稿时间： | 2005-11-08 |
Transfer of Para-carotene Biosynthesis Enzyme Gene(LycB) into Tomato |
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| Kang Yanhong, Ji Jing, Wu Dong,et al..Transfer of Para-carotene Biosynthesis Enzyme Gene(LycB) into Tomato[J].China Vegetables,2006,1(5):11-13. |
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| Authors: | Kang Yanhong Ji Jing Wu Dong |
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| Institution: | Department of Agriculture Sciences; Hebei North University; Zhangjiakou 075131; College of Agriculture and Bioengineering; Tianjin University; Tianjin 300072; Institute of Chemical Defence |
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| Abstract: | The tomato genetic transformation system was established by cocuhivated cotyledons of four varieties of tomato with agrobacterium tumefaciens EHA 101 including the plasmid pEbisLycBHyg. Then LycB gene can be transformed into tomato via agrobacterium tumefaciens. Regeneration plants of tomato were obtained by callus inducing, bud inducing, striking root cultivation and hygromycin screening in selective condition. PCR and PCR-Southern analysis of these resistant plants proved that LycB has been transferred into genome of tomato. The factors affecting genetic transformation were also investigated. |
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| Keywords: | LycB Tomato Cotyledon Transformation |
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