用于黄瓜白粉病抗病性鉴定的SCAR标记

将黄瓜白粉病抗性相关基因的一个共显性AFLP标记成功地转换为简单、实用的SCAR标记。根据AFLP标记片段序列信息设计了两对特异性引物SCPM166和SCPM66。PCR扩增结果显示，SCPM166在抗病亲本、抗病单株和杂合类型单株中均扩增出了大小为166 bp的特异片段，感病亲本与感病单株均无此特异片段，表明该SCAR标记可以鉴定纯合感病个体；SCPM66在感病亲本、感病单株和杂合类型单株中均扩增出了大小为66 bp的特异片段，抗病亲本与抗病单株均无此特异片段，表明该SCAR标记可以鉴定纯合抗病个体。若将两个显性SCAR标记结合起来，相当于一个共显性的SCAR标记。

SCAR Markers for Identification of Cucumber Powdery Mildew Resistance

The co-dominant AFLP marker was successfully converted into simple and applied SCAR markers.Two special SCPM166 and SCPM66 were designed according to the sequence information of AFLP fragments.The SCAR-PCR result showed that the resistant parent,resistant F₂ plants and hybrid plants possessed 166 bp fragment,while the susceptible parent and susceptible F₂ plants did not possess any fragments by primer SCPM166.This indicated that the SCAR marker could identify susceptible homozygous single plant.The susceptible parent,susceptible F₂ plants and hybrid plants possessed 66 bp fragment,while the resistant parent and resistant F₂ plants did not possess any fragments by primer SCPM66.This indicated that the SCAR marker could identify homozygous resistant single plant.By joint application of these 2 dominant SCAR markers,homozygous resistant plant and homozygous susceptible plant could be identified simultaneously.It is equivalent to a commonly dominant SCAR marker.