| 毒杀天牛基因mBt886cry3a转化小黄菊研究 |
| |
| 引用本文： | 周洲,尹新明,卢孟柱,江志伟,王沛. 毒杀天牛基因mBt886cry3a转化小黄菊研究[J]. 北方园艺, 2009, 0(11) |
| |
| 作者姓名： | 周洲 尹新明 卢孟柱 江志伟 王沛 |
| |
| 作者单位： | 河南农业大学,植物保护学院,河南,郑州,450002;河南科技大学,林学院;河南农业大学,植物保护学院,河南,郑州,450002;中国林业科学研究院,林木培育重点实验室,北京,100091 |
| |
| 基金项目： | 河南省杰出人才创新基金资助项目，河南科技大学博士科研启动基金资助项目 |
| |
| 摘 要： | 毒蛋白编码基因 Bt886cry3Aa 对小黄菊进行了遗传转化,转化植株经过PCR和总DNA狭缝杂交验证,得到转基因小黄菊4个样品株.对菊天牛2龄幼虫的生物测定表明:4个样品株毒杀活性明显高于对照,校正死亡率分别为:45.89%、39.12%、35.42%和22.41%.mBt886cry3a转基因植株表现出较强的天牛毒杀活性,为天牛等钻蛀性害虫的防治提供了研究基础.
|
| 关 键 词： | 菊天牛 毒蛋白 遗传转化 小黄菊 |
mBt886cry3a Gene Transformed into Chrysanthemum Dendranthemum grandiflorum 'Xiao Huang' Resistant to The Long Horn Beetles |
| |
| ZHOU Zhou,,YIN Xin-ming,LU Meng-zhu,JIANG Zhi-wei,WANG Pei. mBt886cry3a Gene Transformed into Chrysanthemum Dendranthemum grandiflorum 'Xiao Huang' Resistant to The Long Horn Beetles[J]. Northern Horticulture, 2009, 0(11) |
| |
| Authors: | ZHOU Zhou YIN Xin-ming LU Meng-zhu JIANG Zhi-wei WANG Pei |
| |
| Affiliation: | ZHOU Zhou1,2,YIN Xin-ming1,LU Meng-zhu3,JIANG Zhi-wei1,WANG Pei1(1.Colloge of Plant Protection,Henan Agricultural University,Zhengzhou,Henan 450002,China,2.College of Forestry,Henan University of Science , Technology,Luoyang,Henan 471003,3.Key Laboratory of Forest Silviculture of Forestry CAF,Beijing 100091,China) |
| |
| Abstract: | Bt886Cry3Aa was cloned from Bacillus thuringiensis 886 strain,which was shown toxic to the larva of long horn beetle.Based on the characters of coding usage for pulpous genes,mBt886cry3a was artificially modified and then named mBt886cry3a.Anoplophora glabripennis infests Chrysanthemum Dendrathema grandiflorum 'Xiao Huang' seriously in Henan province.Transgenic approach may be an efficient way to control long horn beetle.In this study,mBt886cry3a were transformed into Chrysanthemum Dendrathema grandiflorum ... |
| |
| Keywords: | Phytoecia rufiventris Bt Agrobacterium tumefaciens-mediated transformation Dendrathema grandiflorum 'Xiao Huang' |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
