| 乙烯处理对玉米胚乳发育过程中淀粉合成的影响 |
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| 引用本文： | 刘晓庆,王萍,詹延廷,王月,杨阳,李昊,董学会. 乙烯处理对玉米胚乳发育过程中淀粉合成的影响[J]. 玉米科学, 2019, 27(1): 54-61,68 |
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| 作者姓名： | 刘晓庆 王萍 詹延廷 王月 杨阳 李昊 董学会 |
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| 作者单位： | 中国农业大学农学院 |
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| 基金项目： | 国家重点研发计划项目子课题（2016YFD0300304） |
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| 摘 要： | 以玉米自交系B73为试验材料,研究施用外源乙烯之后对胚乳内淀粉合成的影响。结果表明,在玉米10展叶时期施用400 mg/L的乙烯利水溶液显著降低玉米百粒重和淀粉含量。淀粉合成相关酶活性测定结果表明,处理组中ADP葡萄糖焦磷酸化酶、可溶性淀粉合成酶、淀粉分支酶、蔗糖合成酶的活性均显著下降。淀粉合成相关基因表达量分析结果表明,处理组中ADP葡萄糖焦磷酸化酶基因ZmSH2、可溶性淀粉合成酶基因ZmSS1、蔗糖合成酶的基因ZmSH1表达量低于对照组。由此推测,外源乙烯通过调控淀粉合成相关基因的表达与酶的活性影响玉米胚乳内淀粉的合成。
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| 关 键 词： | 玉米 胚乳 乙烯 淀粉合成酶 |
Effect of Ethylene on Starch Biosynthesis During Endosperm Development in Maize |
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| LIU Xiao-qing,WANG Ping,ZHAN Yan-ting,WANG Yue,YANG Yang,LI Hao and DONG Xue-hui. Effect of Ethylene on Starch Biosynthesis During Endosperm Development in Maize[J]. Journal of Maize Sciences, 2019, 27(1): 54-61,68 |
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| Authors: | LIU Xiao-qing WANG Ping ZHAN Yan-ting WANG Yue YANG Yang LI Hao DONG Xue-hui |
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| Affiliation: | College of Agronomy, China Agricultural University, Beijing 100193, China,College of Agronomy, China Agricultural University, Beijing 100193, China,College of Agronomy, China Agricultural University, Beijing 100193, China,College of Agronomy, China Agricultural University, Beijing 100193, China,College of Agronomy, China Agricultural University, Beijing 100193, China,College of Agronomy, China Agricultural University, Beijing 100193, China and College of Agronomy, China Agricultural University, Beijing 100193, China |
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| Abstract: | To explore the ethylene effect during starch biosynthesis, maize inbred line B73 was used and sprayed with 400 mg/L ethephon at 10-leaf stage. The results suggested that both 100-kernel weight and starch content decreased significantly after the maize was treated by ethephon solution. Also, the activities of many enzymes that related to starch biosynthesis all decreased significantly, which involved ADP-glucose pyrophosphorylase, soluble starch synthase(SSS), starch branching enzyme(SBE), sucrose synthase(SS), etc. Additionally, we used qRTPCR to analyze the expression patterns of endogenous genes relevant to starch synthesis. The relative expressions of ZmSH2,ZmSS1 and ZmSH1 were all decreased at certain seed development period after exogenous ethylene spraying. These data revealed that ethylene affected starch biosynthesis through influencing enzymes activities and genes expression levels involved in starch synthesis. |
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| Keywords: | Maize Ethylene Endosperm Starch synthase enzyme |
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