| 基于SLAF-seq技术分析甜、糯玉米种质遗传多样性 |
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| 引用本文： | 李余良,索海翠,韩福光,刘建华,胡建广,高磊,李武. 基于SLAF-seq技术分析甜、糯玉米种质遗传多样性[J]. 玉米科学, 2019, 27(4): 71-78 |
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| 作者姓名： | 李余良 索海翠 韩福光 刘建华 胡建广 高磊 李武 |
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| 作者单位： | 广东省农业科学院作物研究所;广东省遗传改良重点实验室 |
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| 基金项目： | 广东省科技计划项目（2016A020208006,2017B090907023）、广东省现代农业共性关键技术项目（2017LM2148）、鲜食玉米现代种业育繁推一体化创新发展联盟建设（粤农计〔2016〕35号）、广东省农业科技创新及推广项目（2018LM2150） |
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| 摘 要： | 利用简化基因组测序技术SLAF-seq,对国内外引进选育的81份鲜食甜、糯玉米自交系进行遗传多样性分析。结果表明,共获得803 058个SLAF标签,其中,多态性SLAF标签373 764个。通过序列分析,获得169 128个有效单核苷酸(SNP)多态标记,利用这些SNP标记分析和构建了81份鲜食甜、糯玉米资源的群体结构和系统发生树,并将其分为2个群。结果表明,简化基因组测序技术SLAF-seq能高效、低廉地开发出大量SNP标记,是作物种质资源群体遗传分析的有效工具。研究结果为甜玉米不同基因的聚合、温-热种质杂交育种、鲜食甜、糯玉米亚种间杂交育种提供依据,有利于鲜食甜、糯玉米自交系的高效利用、品种选育及杂种优势群的建立。
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| 关 键 词： | 甜、糯玉米 种质 SLAF-seq SNPs标记 遗传多样性 |
| 收稿时间： | 2018-08-01 |
Analysis of Genetic Diversity of Sweet and Wax Corn Germplasms Using SLAF-seq Technology |
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| LI Yu-liang,SUO Hai-cui,HAN Fu-guang,LIU Jian-hu,HU Jian-guang,GAO Lei and LI Wu. Analysis of Genetic Diversity of Sweet and Wax Corn Germplasms Using SLAF-seq Technology[J]. Journal of Maize Sciences, 2019, 27(4): 71-78 |
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| Authors: | LI Yu-liang SUO Hai-cui HAN Fu-guang LIU Jian-hu HU Jian-guang GAO Lei LI Wu |
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| Affiliation: | Institute of Crop Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640;Key Laboratory of Crops Genetics and Improvement of Guangdong Province, Guangzhou 510640, China,Institute of Crop Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640;Key Laboratory of Crops Genetics and Improvement of Guangdong Province, Guangzhou 510640, China,Institute of Crop Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640,Institute of Crop Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640,Institute of Crop Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640;Key Laboratory of Crops Genetics and Improvement of Guangdong Province, Guangzhou 510640, China,Institute of Crop Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640;Key Laboratory of Crops Genetics and Improvement of Guangdong Province, Guangzhou 510640, China and Institute of Crop Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640;Key Laboratory of Crops Genetics and Improvement of Guangdong Province, Guangzhou 510640, China |
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| Abstract: | High-throughput specific locus amplified fragment sequencing(SLAF-seq) technology was employed to identify the genetic diversity of 81 inbred lines include sweet and wax corn. The results indicated that the total of 803 058 SLAF tags and 373 764 polymorphic SLAF tags were obtained. A total of 169 128 single nucleotide polymorphisms(SNPs) were obtained through sequences analysis. Population structure and phylogenetic relationship of 81 germplasms of fresh sweet and wax corn were analyzed using the SNP dataset, the population was significantly divided into two different groups. It suggests that SLAF-seq can be used to develop large-scale SNPs for population genetic analysis, effectively and economically. The results can provide molecular basis for gene pyramiding of sweet corn, hybrid breeding of temperate-tropical mode and sweet-wax type. It is beneficial for promoting effective utilization of sweet corn inbred lines, breeding new cultivars and building of heterotic groups. |
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| Keywords: | Sweet and wax corn Germplasm SLAF-seq SNPs marker Genetic diversity |
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