| 广东菠萝心腐病病原鉴定 |
| |
| 引用本文： | 罗建军,刘琼光,何衍彪,李志斌,魏楚丹. 广东菠萝心腐病病原鉴定[J]. 广东农业科学, 2012, 39(13): 90-92 |
| |
| 作者姓名： | 罗建军 刘琼光 何衍彪 李志斌 魏楚丹 |
| |
| 作者单位： | 1. 华南农业大学资源环境学院,广东广州,510642
2. 中国热带农业科学院南亚热带作物研究所,广东湛江,524091 |
| |
| 基金项目： | 国家公益性行业(农业)科技专项 |
| |
| 摘 要： | 为了鉴定广东湛江地区菠萝心腐病病原菌,研究了病原菌的致病性、菌丝体及孢子囊等形态特征,分析了核糖体DNA-ITS序列同源性。结果表明,该病原菌属于疫霉属真菌,同源性分析显示,该菌与GenBank中烟草疫霉ITS序列的同源性达99%以上;采用烟草疫霉特异性引物进行PCR检测,结果确认该病原菌为烟草疫霉菌(Phytophthora nicotianae)。
|
| 关 键 词： | 菠萝 心腐病 病原鉴定 PCR检测 |
Identification of the pathogen causing pineapple heart-rot disease |
| |
| LUO Jian-jun , LIU Qiong-guang , HE Yan-biao , LI Zhi-bin , WEI Chu-dan. Identification of the pathogen causing pineapple heart-rot disease[J]. Guangdong Agricultural Sciences, 2012, 39(13): 90-92 |
| |
| Authors: | LUO Jian-jun LIU Qiong-guang HE Yan-biao LI Zhi-bin WEI Chu-dan |
| |
| Affiliation: | 1(1.College of Natural Resource & Environment,South China Agricultural University,Guangzhou 510642,China;2.South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang 524091,China) |
| |
| Abstract: | In order to identify the pathogen of pineapple leaf heart-rot disease in Guangdong,the fungi was isolated from diseased pineapples.Based on the morphological characteristics,pathogenicity and the sequence of ribosomal DNA-ITS,the pathogen was identified as Phytophthora nicotianae,and the homology analysis showed the sequence of ribosomal DNA-ITS isolated from BL1 had homology of more than 99% with Phytophthora nicotianae published in GenBank.Finally,PCR detection using P.nicotianae specific primers indicated that the pathogen was P.nicotianae. |
| |
| Keywords: | pineapple leaf heart-rot pathogen identification PCR detection |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《广东农业科学》浏览原始摘要信息 |
|
点击此处可从《广东农业科学》下载全文 |
|
