| 中国黑木耳主要栽培菌株酯酶同工酶的研究 |
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| 引用本文： | 唐利华,郭倩,王瑞娟,边银丙. 中国黑木耳主要栽培菌株酯酶同工酶的研究[J]. 食用菌学报, 2007, 14(4): 37-40 |
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| 作者姓名： | 唐利华 郭倩 王瑞娟 边银丙 |
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| 作者单位： | 1. 上海市食用菌工程技术研究中心,上海市农业遗传育种重点开放实验室,上海市农业科学院食用菌研究所,上海,201106
2. 华中农业大学应用真菌研究所,武汉,430070 |
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| 摘 要： | 利用聚丙烯酰胺凝胶垂直板电泳技术对国内34个黑木耳主要栽培菌株进行酯酶同工酶分析,结果表明其酶带数一般为2~6条不等,其中在Rf为0.484处,34个菌株都有谱带出现,34个菌株之间的相似系数在0.143~1.000之间,应用NTSYS软件进行聚类分析结果表明,供试菌株在遗传相似水平为80%时可分六大类,并且根据酯酶同工酶图谱能对部分菌株进行初步鉴别。
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| 关 键 词： | 黑木耳 酯酶同工酶 菌株鉴别 |
| 文章编号： | 1005-9873（2007）04-0037-04 |
| 收稿时间： | 2007-08-20 |
| 修稿时间： | 2007-11-10 |
Esterase Isoenzyme Patterns of the Major Cultivated Auricularia auricula Strains of China |
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| TANG Lihua,GUO Qian,WANG Ruijuan,BIAN Yinbing. Esterase Isoenzyme Patterns of the Major Cultivated Auricularia auricula Strains of China[J]. Acta Edulis Fungi, 2007, 14(4): 37-40 |
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| Authors: | TANG Lihua GUO Qian WANG Ruijuan BIAN Yinbing |
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| Abstract: | Esterase isoenzyme patterns of 34 strains of Auricularia auricula cultivated in China were determined using vertical flat-sheet polyacrylamide gel electrophoresis.Between 2~6 isoenzyme bands were identified from each strain,and a band with an Rf value of 0.484 was detected in all strains.Similarity coefficient values for the 34 strains ranged between 0.143 and 1.000,and the strains were separated into six groups at the level of 80% by NTSYS software. |
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| Keywords: | Auricularia auricula Esterase isoenzyme Strain identification |
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