白僵菌生产中防霉剂的应用研究

为了解决白僵菌固体培养时易遭受霉菌污染问题,以玉米螟白僵菌ABUV–3和松毛虫白僵菌DBUV–7为供试菌株,对40%克菌灵等5种防霉剂进行了系列研究。结果表明:5种防霉剂对毛霉菌、木霉菌、根霉菌、绿霉菌和黄曲霉菌的孢子和菌体均有抑制作用,其中以70%菌绝杀可溶性和绿霉霸粉剂的抑制效果最好;虽然5种防霉剂直接作用时会对白僵菌的孢子萌发产生抑制作用,但在固体培养生产孢子时添加一定量的防霉剂,对孢子产量及其萌发并没有抑制作用。因此,建议在白僵菌生产过程中,在固体培养基中添加70%菌决杀和绿霉霸粉剂500倍液(折合剂量为2 mg/m L),用以抑制霉菌的污染。

Application Research on Mould Inhibitors Production of Beauveria bassiana

In order to solve the problem of Beauveria bassiana vulnerable to mould contamination in solid culture, the corn borer Beauveria bassiana ABUV-3 and the pine caterpillars Beauveria bassiana DBUV-7 as for the tested strains, a series of studies of 40%Kejunling and other ifve kinds of mould inhibitors were conducted. The results showed that the ifve kinds of mould inhibitors on spores and bacteria of Mucor, Trichoderma, Rhizopus, green mould and Aspergillus lfavus all have inhibitory effect, in which the inhibitory effects, the 70% of soluble Junjuesha and Lvmeiba powder was the best; although the direct action of the 5 mould inhibitors will bring inhibited effect on the spore germination ofBeauveria bassiana, there was no inhibitory effect on spore production and germination by adding a certain amount of mould inhibitor when producing spore in solid culture. Therefore, it is suggested that in the process of production ofBeauveria bassiana, in solid culture medium added with 500 times liquid of 70% Junjuesha and Lvmeiba powder (equivalent dose for 2 mg / mL) to inhibit mould contamination.