| 油茶产黄酮内生真菌的初步研究 |
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| 引用本文： | 张婷,左雪枝,马丹.油茶产黄酮内生真菌的初步研究[J].湖南农业科学,2016(11):1-4. |
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| 作者姓名： | 张婷 左雪枝 马丹 |
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| 作者单位： | 荆楚理工学院生物工程学院,湖北荆门,448000 |
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| 基金项目： | 湖北省教育厅科研项目(Q20134301)；校级科研基金项目(ZR201403) |
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| 摘 要： | 试验通过对前期从油茶中分离出的内生真菌进行液体发酵培养、浓缩发酵液等操作获得菌株的次生代谢产物,继而通过盐酸-镁粉颜色反应初筛和薄层层析法复筛,得到2株具有产黄酮能力的内生真菌。这2株内生真菌经形态学鉴定分属于青霉属(Penicillium)和链格孢属(Alternaria),并测得发酵液中的黄酮含量分别为0.011 3和0.013 5 mg/m L。
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| 关 键 词： | 油茶 内生真菌 黄酮 青霉属 链格孢属 |
Primary Studies on Flavonoid-Producing Endophytic Fungi Isolated from Camellia oleifera |
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| Abstract: | Endophytic fungi which were isolated fromCamellia oleifera are cultured in the liquid medium. A lot of secondary metabolites were obtained by condensing the zymotic lfuid and analyzed with color reaction and thin layer chromatography. Eventally 2 endophytic fungi that could produce lfavonoid were obtained and classiifed asPenicillium andAlternaria according to the morphological charactersitic. Furthermore, the lfaconoid content in fermentation liquid were determined as 0.0113 mg/mL and 0.0135 mg/mL respectively. |
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| Keywords: | Camellia oleifera endophytic fungi lfavonoid Penicillium Alternaria |
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