贺兰山地区油松根际固氮菌的多样性研究 |
| |
引用本文: | 牛艳芳,陈立红,闫伟. 贺兰山地区油松根际固氮菌的多样性研究[J]. 湖南林业科技, 2016, 0(5): 17-21. DOI: 10.3969/j.issn.1003-5710.2016.05.004 |
| |
作者姓名: | 牛艳芳 陈立红 闫伟 |
| |
作者单位: | 内蒙古农业大学,内蒙古呼和浩特,010019 |
| |
基金项目: | 国家自然科学基金项目(31260173)。 |
| |
摘 要: | 对贺兰山北寺自然保护区油松(Pinus tabulaeformis)的根际土壤固氮菌多样性和群落结构进行研究。采用阿须贝培养基对土壤中的固氮菌进行分离和纯化,用通用引物27f/1492r对固氮菌进行16S r DNA扩增并测序,应用DNAMAN 6.0软件进行多序列比对,用MEGA 4.0软件构建聚类树。结果表明:从贺兰山北寺油松根际土壤中分离出的固氮菌属于假单胞菌属(Pseudomonas)、芽孢杆菌属(Bacillus)、叶杆菌属(Phyllobacterium)、节杆菌属(Arthrobacter)、根瘤菌属(Rhizobium)、类芽孢杆菌属(Paenibacillus)、鞘氨醇单胞菌属(Sphingomonas)、柄细菌属(Caulobacter)、土地杆菌属(Pedobacter)等9个不同的类群,其中假单胞菌属(Pseudomonas)、芽孢杆菌属(Bacillus)、叶杆菌属(Phyllobacterium)、节杆菌属(Arthrobacter)是优势类群。这表明贺兰山北寺地区油松根际土壤中固氮菌具有较丰富的多样性。
|
关 键 词: | 贺兰山 油松 固氮菌 多样性 |
Diversity of nitrogen-fixing bacteria isolated from Pinus tabulaeformis in Helan Mountains |
| |
Abstract: | The diversity and community structure of nitrogen-fixing bacteria isolated from Pinus tabulaeformis rhizosphere in Helan Mountains was studied. Nitrogen-fixing bacteria from rhizosphere soil were isolated and purified by Ashby’ s Medium,the 16S rDNA gene of nitrogen-fixing bacteria was amplified using primers 27f/1492r and sequenced,the align-ment of 16S rDNA sequences was conducted by DNAMAN 6.0,and the neighbor-joining phylogenetic tree was construc-ted by MEGA 4.0 software. The results showed that nitrogen-fixing bacteria isolated from P. tabulaeformis belonged to nine groups,which were Pseudomonas,Bacillus,Phyllobacterium,Arthrobacter,Rhizobium,Paenibacillus,Sphin-gomonas,Caulobacter,Pedobacter,and Pseudomonas,Bacillus,Phyllobacterium,Arthrobacter were dominant groups.The results indicated that nitrogen-fixing bacteria from P. tabulaeformis in Helan Mountains have rich diversity. |
| |
Keywords: | Helan Mountains Pinus tabulaeformis nitrogen-fixing bacteria diversity |
本文献已被 CNKI 万方数据 等数据库收录! |
|