鸡离体肝细胞几种分离方法的比较

对鸡肝细胞离体培养中几种分离肝细胞的方法进行了比较，试验选用1－56日龄的粤黄鸡，分别采用肝组织块培养、肝组织直接通过细胞筛过滤、2．5g/L胰蛋白酶活消化、2．5g/L胰蛋白酶加0．1g/L EDTA消化、不同时间的4℃冷消化与37℃消化、淋巴细胞分离液分离、5g/L氯化铵除去红细胞等试验方法，观察了鸡离体肝细胞的分离及培养效果，结果表明：采用2．5g/L胰蛋白酶加0．1g/L EDTA 消化10min，用淋巴细胞分离液分离后的肝细胞培养效果最好，肝细胞在培养液中分裂增殖，生长良好。

Comparison of Several Methods for Isolating Chicken Hepatocytes

Comparison was made among several methods for isolating chickenhepatocytes. Yuehuang chicken of 1～56 days old were sacrificed and hepatocytes cultivated from bits of liver tissue, dispersed cell prepared by passing through a cell sieve, digestion with 2.5 g/L pepsin, and digestion with 2.5 g/L pepsin plus 0.1 g/L EDTA. The digestion temperature was 37℃and 4℃. In order to get rid of erythrocytes inside the medium, the reagent for isolating lymphocytes and 5 g/L ammonium chloride were used. The resultes showed that high density and viability of chicken hepatocytes could be obtained after digestion with 2.5 g/L pepsin plus 0.1 g/L EDTA at 37℃ for 10 minutes.