高效液相色谱法测定虎杖中的白藜芦醇含量

[目的]以乙醇为萃取剂,优化提取条件,建立高效液相色谱测定虎杖中的白藜芦醇的方法。[方法]色谱柱为phenomenex C18（250 mm×4.6 mm,5μm）,流动相为无水甲醇-（浓度0.25%）乙酸（65∶35,V/V）等梯度洗脱,流速为0.8 ml/min,检测波长306 nm,以白藜芦醇的提取得率为评价指标,采用单因素试验和L25（5^4）正交试验法筛选最佳工艺条件。[结果]提取白藜芦醇的最佳工艺为：乙醇浓度为60%,料液比为1∶30（g/ml）,萃取温度为55℃,萃取时间为15 min;在此条件下,提取的白藜芦醇的提取得率达到0.826μg/g。[结论]该方法简便、快捷、准确性高,适用于虎杖中的白藜芦醇的测定。

Determination of Resveratrol Content in RHIZOMA ET RADIX POLYGONI CUSPIDATI by HPLC

[Objective] Based on optimizing a variety of experimental conditions,a method for the determination of resveratrol in RHIZOMA ET RADIX POLYGONI CUSPIDATI by HPLC was established by using ethanol as extractant.[Method] Phenomenex C18(250 mm×4.6 mm,5 μm) was selected as column,with methanol-0.25% acetic acid(65∶ 35,V/V) as mobile phase,0.8 ml/min mobile speed and 306 nm detection wavelength.Taking the extraction rate of resveratrol as the evaluation index,single factor test and L25(54) orthogonal test were used to optimize the experimental conditions.[Result] The extraction conditions for resveratrol were 60% ethanol,1∶ 30(g/ml) solid-liquid ratio,55 ℃ extraction temperature and 15 min extraction time.Under this condictions,the extraction rate of resveratrol reached 0.826 μg/g.[Conclusion] This method was proved to be simple,rapid,accurate and could be used for the determination of resveratrol content in RHIZOMA ET RADIX POLYGONI CUSPIDATI.