同化玉米芯酸解液为丁醇

目的]探究用于丙酮丁醇发酵的玉米芯酸解液的制备方法,寻找培养基组分的较佳组合。方法]以产物丁醇浓度为指标,比较4种不同玉米芯酸解液制备方法。结果]稀硫酸处理玉米芯后,用Ca（OH）2调节pH,经XAD-4树脂、活性炭处理,可得到13.71 g/L丁醇;利用薄层层析法分析酸解液中主要还原糖是葡萄糖和木糖,经发酵分析可为Clostridium acetobutylicum ATCC824相同利用;经单因素分析,培养基组分较佳组合为：100%玉米芯水解液中,需加入2 g/L的FeSO4,30 g/L的黄豆饼粉,得到丁醇的最高浓度为19.2 g/L。结论]玉米芯酸解液可用于丁醇发酵,但需去除有毒物。

Assimilation and Conversion Acid Hydrolysis Solution of Corn Cob into Butanol

Objective] The preparation method of corn cob acid hydrolysis solution for the acetone-butanol fermentation was studied in order to find a better combination of medium components.Method] With butanol concentration as index,four different preparation methods for corn cob acid hydrolysis solution were compared.Result] The results indicated that the concentration of butanol was 13.71 g/L,when the pH was adjusted by Ca(OH)2 after pretreated by sulfuric acid,followed by adsorption of inhibitors with XAD-4 resin and activated carbon.The main monosaccharide analyzed by Thin-Layer Chromatography(TLC)was glucose and xylose in the acid hydrolysis solution.The two kind of monosaccharide could be the same used as carbon source for butanol fermentation by Clostridium acetobutylicum ATCC824.The result of the single factor experiment was that,the best concentration of butanol was 19.2 g/L when 100% acid hyrolysis solution was added FeSO4(2 g/L) and soybean cake meal(30 g/L).Conclusion] The acid hydrolysis solution of corncob could be used in butanol fermentation when the inhibitors were reduced.