野生木薯组织培养快繁技术研究

目的]对野生木薯进行种质资源保存和大规模种苗繁殖。方法]采用组织培养的方法进行快速繁殖。结果]适宜野生木薯愈伤与不定芽分化的培养基为MS＋6-BA 0.10～0.50 mg/L＋NAA 0.1～0.5 mg/L;继代增殖适宜培养基为MS（Fe含量加倍）＋6-BA 0.50～0.65 mg/L＋IBA 0.1 mg/L＋PVP 200 mg/L;适宜生根的培养基为MS（Fe含量加倍）＋0.1%AC,生根率达90%以上;在粗河沙∶细河沙=2∶1基质中驯苗,成活率可达85%以上。结论]建立了野生木薯的快速繁殖方法。

Study on Tissue Culture and Rapid Propagation Technique for Wild Cassava

Objective] The study aimed to conduct the germplasm resource conservation and large-scale seedling propagation on the wild cassava.Method] The tissue culture method was use to make for the rapid propagation.Result] The medium suitable for the callus induction and the adventitious bud differentiation of the wild cassava was MS+6-BA 0.10-0.50 mg/L+NAA 0.1-0.5 mg/L;the medium suitable for the proliferation was MS(Fe content doubling)+6-BA 0.50-0.65 mg/L+IBA 0.1 mg/L+PVP 200 mg/L;the medium suitable for rooting was MS(Fe content doubling)+0.1% AC and the rooting rate was more than 90%;when the test tube seedlings was made for the domestication in the matrix with the ratio of the crud river sand to the fine river sand of 2∶ 1,the survival rate was 85%.Conclusion] The rapid propagation method for the wild cassava was established.