基于ITS序列云南老虎须(Tacca chantrieri)居群遗传多样性的探讨

[目的]研究9个云南老虎须居群的亲缘关系,为该种质资源的合理利用和保护提供理论依据。[方法]利用ITS序列分析技术,对采自云南省的9个老虎须居群共118个样品进行遗传多样性研究。[结果]9个老虎须居群的样品的ITS序列被分成了2个大组,滇南4个居群聚为一组,滇东南的5个居群聚为另一组。9个居群共6个单倍型,两组之间无重合单倍型。[结论]老虎须的遗传多样性很低,遗传变异主要存在于居群之间。这可能是因为云南分布的老虎须是以自交为主的植物,因此对老虎须进行迁地保护时应该是尽量引种多个居群。

Discussion on Genetic Diversity of Tacca chantrieri Population in Yunnan Province Based on ITS Sequence Analysis

[Objective] The study aimed to investigate the genetic relationship among 9 populations of Tacca chantrieri in Yunnan Province and provide the theoretical basis for the rational utilization and protection of the germplasm resources.[Method] The ITS sequence analysis technology was used to make for the research on the genetic diversity of 118 samples collected from 9 populations of Tacca chantrieri in Yunnan Province.[Result] The samples from 9 populations were divided into 2 groups: one included 4 populations from the south of Yunnan Province;another included the rest 5 populations from the southeast of Yunnan Province.There were 6 haplotypes in 9 populations and there was noncoincidence haplotype between 2 groups.[Conclusion] T.chantrieri showed a very low genetic diversity,the genetic variation was mainly among populations.This may be attributed to that the T.chantrieri distributed in Yunnan Province is the plant with the self-pollination as the main.Therefore,the more populations should be introduced as far as possible when T.chantrieri was made for the off-site preservation.