| 板蓝根饮片糖基化蛋白纳米颗粒的构建与表征 |
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| 引用本文： | 查飞,叶海峰,梁剑,朱品玲,钟茂生,江晓芬. 板蓝根饮片糖基化蛋白纳米颗粒的构建与表征[J]. 安徽农业科学, 2011, 39(34): 21007-21009 |
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| 作者姓名： | 查飞 叶海峰 梁剑 朱品玲 钟茂生 江晓芬 |
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| 作者单位： | 中华人民共和国宁德出入境检验检疫局,福建宁德,352100;中华人民共和国宁德出入境检验检疫局,福建宁德,352100;中华人民共和国宁德出入境检验检疫局,福建宁德,352100;中华人民共和国宁德出入境检验检疫局,福建宁德,352100;中华人民共和国宁德出入境检验检疫局,福建宁德,352100;中华人民共和国宁德出入境检验检疫局,福建宁德,352100 |
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| 摘 要： | 从板蓝根饮片中分离出糖基化蛋白,体外构建纳米颗粒,并对所形成胶体的理化性质和生物活性进行表征。经SDS-PAGE鉴定板蓝根饮片粗提液中存在3个主要的糖基化蛋白,用这些蛋白构建的纳米颗粒的粒径约为50 nm。MDCK细胞试验表明,蛋白纳米颗粒具有较强的抗病毒能力。
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| 关 键 词： | 板蓝根 糖基化蛋白 纳米颗粒 表征 |
Construction and Characterization of Glycosylated Proteins Nano-particles in Radix Isatidis |
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| Affiliation: | CHA Fei et al(Ningde Import and Export Inspection and Quarantine Bureau of PRC,Ningde,Fujian 352100) |
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| Abstract: | The glycosylated proteins was isolated from radix isatidis for constructing nano-particles in vitro,the physicochemical properties and biological activity of the colloid was characterized.The appraisal result of SDS-PAGE showed that there were 3 kinds of glycosylated proteins in crude extract of radix isatidis,and the particle size of the nano-particles constructed by those proteins was 50 nm.The results of MDCK cell test showed that the glycosylated proteins nano-particles had strong antiviral ability. |
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| Keywords: | Radix isatidis Glycosylated proteins Nano-particles Characterization |
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