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鸡白痢沙门氏菌的分离与鉴定
引用本文:安辉,张大华,陈小玲,徐福洲,贺云霞,黄秀芬,李桂萍,孙惠玲.鸡白痢沙门氏菌的分离与鉴定[J].安徽农业科学,2011,39(32):19888-19890.
作者姓名:安辉  张大华  陈小玲  徐福洲  贺云霞  黄秀芬  李桂萍  孙惠玲
作者单位:1. 北京市农林科学院畜牧兽医研究所,北京100097;内蒙古民族大学生命科学学院,内蒙古通辽028000
2. 山东省潍坊市寒亭区畜牧兽医局,山东潍坊,261100
3. 北京市农林科学院畜牧兽医研究所,北京,100097
基金项目:国家自然科学基金项目(30871867);北京市农林科学院青年基金项目(QNJJ201012)
摘    要:目的]探索20日龄雏鸡出现有关节肿胀、拉白色稀粪和少量死亡等症状的疾病病因,为鸡场的疾病诊断和防治提供建议和有效措施。方法]采集病鸡样品并进行了细菌学检验。通过细菌分离、PCR、生化试验和回鸡试验鉴定出5株鸡白痢沙门氏菌。结果]用分离株感染SPF雏鸡,能复制出与自然感染一致的病例。结论]鸡白痢沙门氏菌是造成鸡场该次疾病的主要病原。

关 键 词:鸡白痢  鸡白痢沙门氏菌  实验室诊断

Isolation and Identification of Salmonella Pullorum
Institution:AN Hui et al(Institute of Animal Husbandry and Veterinary Medicine,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097)
Abstract:Objective] The disease of 20-day-old chick with the character of swell-joint and blanc-loose stool resulting in few death was analyzed for the provision of the effective measurement of its diagnosis and control.Method] The bacteriological character of the sample from the diseased chick was tested and 5 strains of Salmonella Pullorum from the sample were identified through bacterial isolation,PCR,biochemical test and its inoculation experiment.Results] The chicks could be infected by the isolated strain,which were similar clinical signs as the diseased chick in farm.Conclusion] The pathogen of Salmonella Pullorum was main reason in the chick-diseasing.
Keywords:Pullorum disease  Salmonell Pullorum  Diagnosis in laboratory
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