抗双酚A多克隆抗体的制备 |
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引用本文: | 匡华,刘丽强,胡拥明,宋珊珊,李伟峰,胥传来. 抗双酚A多克隆抗体的制备[J]. 安徽农业科学, 2011, 39(34): 21353-21354,21356 |
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作者姓名: | 匡华 刘丽强 胡拥明 宋珊珊 李伟峰 胥传来 |
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作者单位: | 江南大学食品学院,江苏无锡,214122 |
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基金项目: | 质检公益性行为科研专项 |
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摘 要: | [目的]制备可以识别双酚A(BPA)的多克隆抗体。[方法]通过双酚酸(DPA)与载体蛋白牛血清白蛋白(BSA)以及卵清白蛋白(OVA)偶联制备免疫抗原DPA-BSA和包被抗原DPA-OVA,以DPA-BSA免疫新西兰大白兔获得抗血清,并采用间接竞争酶联免疫吸附(ELISA)检测方法对抗体进行鉴定。[结果]经紫外光谱分析,DPA-BSA和DPA-OVA制备成功。抗血清效价最高达到1∶256 000,50%抑制率(IC50)达17.2 ng/ml。[结论]该研究为建立双酚A的快速筛选方法奠定了基础。
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关 键 词: | 双酚A 抗血清 多克隆抗体 ELISA |
Preparation of Polyclonal Antibody of Anti-bisphenol A |
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Affiliation: | KUANG Hua et al(School of Food Science and Technology,Jiangnan University,Wuxi,Jiangsu 214122) |
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Abstract: | [Objective] The paper aimed to prepare polyclonal antibody that could recognize Bisphenol A.[Method] Diphenolic acid(DPA)was used to couple with carrier protein BSA and OVA for preparing immune antigen DPA-BSA and coating antigen DPA-OVA,and the New Zealand white rabbits were immunized with DPA-BSA to prepare antiserum.The antibody was determined by indirect competitive enzyme-linked immunosorbent assay(ELISA).[Result] UV-Spectroscopic analysis showed that DPA-BSA and DPA-OVA were successfully prepared.The antiserum titer was as high as 1∶ 256 000,and 50% inhibitory concentration was 17.2 ng/ml.[Conclusion] The study lays a foundation for developing rapid detection method of BPA. |
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Keywords: | Bisphenol A Antiserum Polyclonal antibody ELISA |
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